Effects of nitrogen and phosphorus rates, leaf age and drying technique on growth and nutritional contents of Moringa oleifera
- Authors: Sokombela, Asanda
- Date: 2019
- Subjects: Moringa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12117 , vital:39153
- Description: In general, smallholder farmers tend to use large quantities of soil nutrients without replenishment with manure, compost and fertiliser in order to replace the soil nutrients that are lost. Considering the low soil fertility status of soils in South Africa, this study was conducted with an aim of developing agronomic protocols for the production of moringa in order to fight malnutrition and produce supplement feed for animals. Moringa contains various nutrients and chemicals, such as, protein, which are sensitive to heat, and there is therefore a need to establish the best drying method which does not affect the nutrient content and quality of moringa. Physiological age is probably the most important factor influencing the mineral composition of plants. The relationship between nutrient concentration and leaf age is not fundamentally altered by crop load, soil type, climate or cultural practices, such that nutrients may be displaced vertically by the level of supply. Leaf age is one of the factors that affect the distribution of nutrients in the leaves of plants. Therefore, studies were conducted to evaluate the response of Moringa oleifera leaf yield and nutritional content to nitrogen and phosphorus rates, leaf age/position, and drying techniques. The experiments were conducted at the University of Fort Hare Alice Campus research farm. The experiment that investigated the effect of N and P rates was carried out in potted plants filled fine silica sand. The seeds were sown on black polythene bags, filled with a mixture of soil, pine bark and goat manure (at 1:2:1 ratio, respectively) at a depth of 2 cm. At 30 cm height, the seedlings were transplanted into 5-liter pots, which were filled with building sand as growing media. The experiment was a 4 x 3 factorial, with four levels of nitrogen (100, 200, 300 and 400 kg/ha) and three levels of phosphorus (40, 80 and 120 kg/ha). The experiment was laid-out as randomised complete block design (RCBD), with 12 treatment combinations replicated four times. All experimental units received the same amount of potassium (731kg/ha) in the form of KCl. Nitrogen treatments which were applied were split in two equal applications, at transplanting stage and 4 weeks later. The phosphorus rates were applied as superphosphate once mixed to the potting media before transplanting. Growth parameters such as plant height, root collar diameters, number of leaves, dry and fresh leaf masses were measured. Powdered dry leaves were also analysed to determine iv metabolite such as crude protein, ash, moisture, fat and fibre constituents (ADF and NDF). Micro and macro mineral nutrients were also determined using atomic absorption spectroscopy technique (AAS). The results showed that growth of moringa significantly increased through the application of fertilizer. The results from the (ANOVA) table showed no interaction between phosphorus and nitrogen on growth parameters such as number of leaves, stem width and plant height of moringa in this study. Nitrogen levels, as treatments, have significantly affected the growth of the plant. However, phosphorus, as a treatment, showed no significant difference on growth parameteres of Moringa. It was evident that trees that received the highest nitrogen rate (400 kg/ha) resulted in thickest stem girth, tallest trees and the highest number of leaves. The overall results revealed that moringa leaf powder contained appreciable amount nutrients. Crude protein was notably high (38.23%) in the trees that received the highest phosphorus and nitrogen combination treatment (400 kg/ha N x 120 kg/ha P treatment combination. The highest ash content of (14.17%) and (14.21%) were recorded from the trees that received treatment combination of 200 kg/ha N x 80 kg/ha P and 100 kg/ha N x 120 kg/ha P, and significantly differed from the rest of the other treatment combinations. The fat content steadily increased with an increase in nitrogen rate. Increase in P level resulted in a decrease in fat content. The overall findings confirm that combinations of the higher levels of N and P result in a decrease in fat content. The variation in the metabolite nutrients and mineral nutrient values may be due to differences in age of the leaves or positions and possibly due to different stages of maturity of leaves. The second experiment was conducted in the field at the research farm. The aim was to evaluate the effect of leaf age/position on nutrient distribution of moringa. The seeds were sown in black polythene bags at a depth of 2 cm. The bags were filled with a mixture of 1 part of soil, 2 parts of pine bark: and 1 part of manure. The seedlings were transplanted into the field when they reached the height of 30cm. Plants were laid-out as a CRD design with 5 replications. Three leaf positions, upper, middle and basal leaves, were used as treatments to determine the nutrient variation within a parent plant. Leaves of each position were harvested separately, washed with tap water and air-dried in shade condition. The dried leaves were analysed for metabolite and mineral content. The Association of official agricultural chemists (AOAC) methods were used to determine protein, fat, fibre, and ash. Atomic absorption spectrometry (AAS) technique was used to analyse minerals v such as iron, zinc, copper, manganese and macro-minerals such as calcium, magnesium, potassium, sodium and phosphorus. The study showed that ash, fat, NDF and ADF contents were significantly higher in the basal leaves as compared to that in the middle and upper leaves. However, moisture and crude protein contents were significantly higher in the upper leaves compared to both in the middle and basal leaves. The basal leaves accumulated significantly higher Ca, Mg, K, Na and Mn contents. The concentrations of P, Zn, Cu and Fe were, on the other hand, significantly higher in the younger leaves compared to that in the middle and basal leaves. It can be concluded that the upper (younger) leaves contain enough nutrition to satisfy the dietary requirements. In addition, these leaves have low levels of oxalate, cyanide and nitrate, which can cause health problems. To determine the effect of drying method on nutrition content of moringa leaves, three drying methods (shade-, sun- and oven-drying methods) were applied as treatments. Moringa plants were grown at the Research Farm of the University of Fort Hare, Alice campus. The leaves were harvested, thoroughly mixed, washed with tap water and put into 15 brown paper bags. The 15 samples (paper bags) were distributed to the three drying treatments (each treatment replicated 5 times). The dried samples were analysed for metabolites and mineral content as per AOAC and AAS methods, respectively. The study showed that shade-drying method retained higher metabolites. Thus, this study recommends the shade-drying method in preserving moringa leaves.
- Full Text:
- Date Issued: 2019
- Authors: Sokombela, Asanda
- Date: 2019
- Subjects: Moringa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12117 , vital:39153
- Description: In general, smallholder farmers tend to use large quantities of soil nutrients without replenishment with manure, compost and fertiliser in order to replace the soil nutrients that are lost. Considering the low soil fertility status of soils in South Africa, this study was conducted with an aim of developing agronomic protocols for the production of moringa in order to fight malnutrition and produce supplement feed for animals. Moringa contains various nutrients and chemicals, such as, protein, which are sensitive to heat, and there is therefore a need to establish the best drying method which does not affect the nutrient content and quality of moringa. Physiological age is probably the most important factor influencing the mineral composition of plants. The relationship between nutrient concentration and leaf age is not fundamentally altered by crop load, soil type, climate or cultural practices, such that nutrients may be displaced vertically by the level of supply. Leaf age is one of the factors that affect the distribution of nutrients in the leaves of plants. Therefore, studies were conducted to evaluate the response of Moringa oleifera leaf yield and nutritional content to nitrogen and phosphorus rates, leaf age/position, and drying techniques. The experiments were conducted at the University of Fort Hare Alice Campus research farm. The experiment that investigated the effect of N and P rates was carried out in potted plants filled fine silica sand. The seeds were sown on black polythene bags, filled with a mixture of soil, pine bark and goat manure (at 1:2:1 ratio, respectively) at a depth of 2 cm. At 30 cm height, the seedlings were transplanted into 5-liter pots, which were filled with building sand as growing media. The experiment was a 4 x 3 factorial, with four levels of nitrogen (100, 200, 300 and 400 kg/ha) and three levels of phosphorus (40, 80 and 120 kg/ha). The experiment was laid-out as randomised complete block design (RCBD), with 12 treatment combinations replicated four times. All experimental units received the same amount of potassium (731kg/ha) in the form of KCl. Nitrogen treatments which were applied were split in two equal applications, at transplanting stage and 4 weeks later. The phosphorus rates were applied as superphosphate once mixed to the potting media before transplanting. Growth parameters such as plant height, root collar diameters, number of leaves, dry and fresh leaf masses were measured. Powdered dry leaves were also analysed to determine iv metabolite such as crude protein, ash, moisture, fat and fibre constituents (ADF and NDF). Micro and macro mineral nutrients were also determined using atomic absorption spectroscopy technique (AAS). The results showed that growth of moringa significantly increased through the application of fertilizer. The results from the (ANOVA) table showed no interaction between phosphorus and nitrogen on growth parameters such as number of leaves, stem width and plant height of moringa in this study. Nitrogen levels, as treatments, have significantly affected the growth of the plant. However, phosphorus, as a treatment, showed no significant difference on growth parameteres of Moringa. It was evident that trees that received the highest nitrogen rate (400 kg/ha) resulted in thickest stem girth, tallest trees and the highest number of leaves. The overall results revealed that moringa leaf powder contained appreciable amount nutrients. Crude protein was notably high (38.23%) in the trees that received the highest phosphorus and nitrogen combination treatment (400 kg/ha N x 120 kg/ha P treatment combination. The highest ash content of (14.17%) and (14.21%) were recorded from the trees that received treatment combination of 200 kg/ha N x 80 kg/ha P and 100 kg/ha N x 120 kg/ha P, and significantly differed from the rest of the other treatment combinations. The fat content steadily increased with an increase in nitrogen rate. Increase in P level resulted in a decrease in fat content. The overall findings confirm that combinations of the higher levels of N and P result in a decrease in fat content. The variation in the metabolite nutrients and mineral nutrient values may be due to differences in age of the leaves or positions and possibly due to different stages of maturity of leaves. The second experiment was conducted in the field at the research farm. The aim was to evaluate the effect of leaf age/position on nutrient distribution of moringa. The seeds were sown in black polythene bags at a depth of 2 cm. The bags were filled with a mixture of 1 part of soil, 2 parts of pine bark: and 1 part of manure. The seedlings were transplanted into the field when they reached the height of 30cm. Plants were laid-out as a CRD design with 5 replications. Three leaf positions, upper, middle and basal leaves, were used as treatments to determine the nutrient variation within a parent plant. Leaves of each position were harvested separately, washed with tap water and air-dried in shade condition. The dried leaves were analysed for metabolite and mineral content. The Association of official agricultural chemists (AOAC) methods were used to determine protein, fat, fibre, and ash. Atomic absorption spectrometry (AAS) technique was used to analyse minerals v such as iron, zinc, copper, manganese and macro-minerals such as calcium, magnesium, potassium, sodium and phosphorus. The study showed that ash, fat, NDF and ADF contents were significantly higher in the basal leaves as compared to that in the middle and upper leaves. However, moisture and crude protein contents were significantly higher in the upper leaves compared to both in the middle and basal leaves. The basal leaves accumulated significantly higher Ca, Mg, K, Na and Mn contents. The concentrations of P, Zn, Cu and Fe were, on the other hand, significantly higher in the younger leaves compared to that in the middle and basal leaves. It can be concluded that the upper (younger) leaves contain enough nutrition to satisfy the dietary requirements. In addition, these leaves have low levels of oxalate, cyanide and nitrate, which can cause health problems. To determine the effect of drying method on nutrition content of moringa leaves, three drying methods (shade-, sun- and oven-drying methods) were applied as treatments. Moringa plants were grown at the Research Farm of the University of Fort Hare, Alice campus. The leaves were harvested, thoroughly mixed, washed with tap water and put into 15 brown paper bags. The 15 samples (paper bags) were distributed to the three drying treatments (each treatment replicated 5 times). The dried samples were analysed for metabolites and mineral content as per AOAC and AAS methods, respectively. The study showed that shade-drying method retained higher metabolites. Thus, this study recommends the shade-drying method in preserving moringa leaves.
- Full Text:
- Date Issued: 2019
Nutritional and pharmacological profile of moringa oleifera lam. Leaves cultivated in the South African ecotype
- Authors: Yako, Zomsa
- Date: 2019
- Subjects: Moringa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14605 , vital:40021
- Description: Moringa oleifera Lam. is native to the Indian ecotype, which has become naturalized in many tropic and subtropic regions worldwide, thus a great variation in the bioactive compounds of the plant is recorded. The introduction of Moringa oleifera to South Africa occurred in the turn of the twentieth century with the aim of combating malnourishment, aiding health and fostering economic development. Therefore, this study was conducted with the aim of evaluating and authenticating the nutritional parameters, essential oil and bioactive components found in the various solvent extracts of Moringa oleifera leaves cultivated in the South African ecotype. It also elucidated some of the plants medicinal properties by investigating the antioxidant and antibacterial properties of the plant. The essential oil of the leaves was extracted using two methods (solvent free microwave extraction and hydrodistillation) and analysed using Gas Chromotography/Mass Spectrometer. The safety profile of the leaves was investigated using brine shrimp model. The results from the study revealed that Moringa oleifera leaves cultivated in South Africa were rich in crude protein levels (28.72%) and carbohydrate (28.84%). Among the minerals tested for, the leaves contained high content of calcium (1603.33 mg/100 g), potassium (1690 mg/100 g), zinc (13.03 mg/100 g) and iron (21.13 mg/100 g). The vitamin E content had the highest concentration (89.43 mg/100 g) among the vitamins evaluated. The leaves of Moringa oleifera had low quantities of antinutrients in relation to minerals, thus ensuring nutrients availability. This study identified 15 fatty acids in the dried leaves of Moringa. The highest fatty acid detected were polyunsaturated fatty acids, linoleic acid (56.36) and alpha-linolenic acid (687.58), 7 of the fatty acids were saturated fatty acids, though relatively low besides stearic acid. 15 amino acids were present, 7 of which were essential amino acids, namely; threonine, tyrosine, methionine, valine, phenylalanine, isoleucine and leucine. vi Among the phytochemicals evaluated, the aqueous extract gave the highest total phenolic content (21.01 ± 2.315 mg GAE/g), the acetone extract gave the highest proanthocyanidin (15.33 3.5 mg CE/g) and flavonoid contents (25.04±3.28 mg QE/g), while saponin and alkaloid contents were 27.73 ± 9.97% and 7.32 ± 2.73 % respectively. All the extracts exhibited high radical scavenging power against the 2,2- diphenyl-1-picrylhydrazyl radical with an IC50 less than 0.025 mg/mL. Aqueous extract gave the highest capacity based on ABTS and TAC assays with IC50 of 0.074 mg/ mL and 0.055 mg/mL respectively. Ethanol extract exhibited the least antioxidant capacity among the extracts, as well as the least antimicrobial activity on both Gram −ve and Gram +ve bacteria with all its minimum inhibitory concentration values greater than 5 mg/mL. The acetone extract exhibited the strongest antimicrobial activity among the extracts. A total of 8 and 24 chemical compounds were found from the solvent-free microwave extracted and hydrodistilled oils, respectively. The major components found from hydrodistillation were 2-Hexanal (16.37%), Nonanal (6.09%), 5 9-undecadien-2-one 6, 10 dimethyl- (e) - (8.01%), trans-beta-Ionone (8.02%) and octadecane (9.67%). For the solvent free microwave extracted oil, Benzene acetaldehyde (5.68%), Octadecane, Pentacosane and Eicosane which were 11.03%, were the major components. Toxicity evaluation revealed that mortality was time dependent. As the exposure period increased, mortality kept increasing. The hatching success of the cysts in acetone, aqueous and ethanol extracts were 35.7, 33.6 and 35.2% respectively. Acetone extract showed the most prominent hatching success. Hatchability and lethality were in a concentration dependent fashion. Among the extracts, acetone extract exhibited the highest lethality (12.3%) to the nauplii, followed by ethanol (10.9%), then aqueous (5.6%) extracts. All the results obtained from this study suggest that Moringa has the potential of eradicating malnutrition and aid health. It contains the necessary nutritional and bioactive compounds and the leaves proved not to be toxic at the levels (0.0625 – 1 mg/mL) tested.
- Full Text:
- Date Issued: 2019
- Authors: Yako, Zomsa
- Date: 2019
- Subjects: Moringa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14605 , vital:40021
- Description: Moringa oleifera Lam. is native to the Indian ecotype, which has become naturalized in many tropic and subtropic regions worldwide, thus a great variation in the bioactive compounds of the plant is recorded. The introduction of Moringa oleifera to South Africa occurred in the turn of the twentieth century with the aim of combating malnourishment, aiding health and fostering economic development. Therefore, this study was conducted with the aim of evaluating and authenticating the nutritional parameters, essential oil and bioactive components found in the various solvent extracts of Moringa oleifera leaves cultivated in the South African ecotype. It also elucidated some of the plants medicinal properties by investigating the antioxidant and antibacterial properties of the plant. The essential oil of the leaves was extracted using two methods (solvent free microwave extraction and hydrodistillation) and analysed using Gas Chromotography/Mass Spectrometer. The safety profile of the leaves was investigated using brine shrimp model. The results from the study revealed that Moringa oleifera leaves cultivated in South Africa were rich in crude protein levels (28.72%) and carbohydrate (28.84%). Among the minerals tested for, the leaves contained high content of calcium (1603.33 mg/100 g), potassium (1690 mg/100 g), zinc (13.03 mg/100 g) and iron (21.13 mg/100 g). The vitamin E content had the highest concentration (89.43 mg/100 g) among the vitamins evaluated. The leaves of Moringa oleifera had low quantities of antinutrients in relation to minerals, thus ensuring nutrients availability. This study identified 15 fatty acids in the dried leaves of Moringa. The highest fatty acid detected were polyunsaturated fatty acids, linoleic acid (56.36) and alpha-linolenic acid (687.58), 7 of the fatty acids were saturated fatty acids, though relatively low besides stearic acid. 15 amino acids were present, 7 of which were essential amino acids, namely; threonine, tyrosine, methionine, valine, phenylalanine, isoleucine and leucine. vi Among the phytochemicals evaluated, the aqueous extract gave the highest total phenolic content (21.01 ± 2.315 mg GAE/g), the acetone extract gave the highest proanthocyanidin (15.33 3.5 mg CE/g) and flavonoid contents (25.04±3.28 mg QE/g), while saponin and alkaloid contents were 27.73 ± 9.97% and 7.32 ± 2.73 % respectively. All the extracts exhibited high radical scavenging power against the 2,2- diphenyl-1-picrylhydrazyl radical with an IC50 less than 0.025 mg/mL. Aqueous extract gave the highest capacity based on ABTS and TAC assays with IC50 of 0.074 mg/ mL and 0.055 mg/mL respectively. Ethanol extract exhibited the least antioxidant capacity among the extracts, as well as the least antimicrobial activity on both Gram −ve and Gram +ve bacteria with all its minimum inhibitory concentration values greater than 5 mg/mL. The acetone extract exhibited the strongest antimicrobial activity among the extracts. A total of 8 and 24 chemical compounds were found from the solvent-free microwave extracted and hydrodistilled oils, respectively. The major components found from hydrodistillation were 2-Hexanal (16.37%), Nonanal (6.09%), 5 9-undecadien-2-one 6, 10 dimethyl- (e) - (8.01%), trans-beta-Ionone (8.02%) and octadecane (9.67%). For the solvent free microwave extracted oil, Benzene acetaldehyde (5.68%), Octadecane, Pentacosane and Eicosane which were 11.03%, were the major components. Toxicity evaluation revealed that mortality was time dependent. As the exposure period increased, mortality kept increasing. The hatching success of the cysts in acetone, aqueous and ethanol extracts were 35.7, 33.6 and 35.2% respectively. Acetone extract showed the most prominent hatching success. Hatchability and lethality were in a concentration dependent fashion. Among the extracts, acetone extract exhibited the highest lethality (12.3%) to the nauplii, followed by ethanol (10.9%), then aqueous (5.6%) extracts. All the results obtained from this study suggest that Moringa has the potential of eradicating malnutrition and aid health. It contains the necessary nutritional and bioactive compounds and the leaves proved not to be toxic at the levels (0.0625 – 1 mg/mL) tested.
- Full Text:
- Date Issued: 2019
Effects of dietary inclusion of Moringa oleifera leaf meal on growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork
- Authors: Nduku, Xola Pauline
- Date: 2014
- Subjects: Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11836 , http://hdl.handle.net/10353/d1021281 , Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Description: The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.
- Full Text:
- Date Issued: 2014
- Authors: Nduku, Xola Pauline
- Date: 2014
- Subjects: Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11836 , http://hdl.handle.net/10353/d1021281 , Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Description: The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.
- Full Text:
- Date Issued: 2014
Effects of feeding Moringa Oleifera leaf meal as an additive on growth performance of chicken, physico-chemical shelf-life indicators, fatty acid profiles and lipid oxidation of broiler meat
- Authors: Wapi, Cwayita
- Date: 2012
- Subjects: Moringa , Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11822 , http://hdl.handle.net/10353/d1007546 , Moringa , Broilers (Chickens) , Meat -- Quality
- Description: Effects of feeding Moringa oleifera leaf meal as an additive on growth performance of chicken, physico- chemical shelf-life indicators, fatty acids profiles and lipid oxidation of broiler meat The main objective of the study was to determine the effect of M.oleifera leaf meal (MOLM) as an additive on growth performance, carcass characteristics, physico-chemical shelf-life indicators (colour, ultimate pH, driploss), fatty acids profiles and lipid oxidation of meat from broilers. A total of 432 1day old unsexed broiler chicks (Aviane 48) were randomly allocated to four dietary treatments (TRTS) in 72 cages. There were 18 cages per treatment and each cage allocated 6 chicks. Water and feed was provided at ad libitum. The feeding phases were, prestarter (0-7 Days), starter (8-18 Days), grower (19-28 Days), finisher (29-35 Days). The four TRTS contained graded levels of MOLM at 1000g/ton, 750g/ton, 500g/ton, and 0g/ton (control), respectively. The birds were slaughtered at 35 days of age. Breast muscles were sampled for meat, ultimate pH (pHu ), colour, drip loss over a 7 days shelf-life test. After each day’s test sub-samples were dipped in liquid nitrogen and kept at -180 C for thiobarbituric acid reactive substances determination. On Day1 and Day 7 extra sub-samples were also kept at -180 C for fatty acids analysis.The TRTS had no effect on average feed intake (AFI), feed conversion efficiency (FCE), and on average daily gain (ADG). Slaughter weight (SW), carcass weight (CW), dressing percentage and gizzard weight (GW) values were similar in all TRTS. Liver weight (LW), heart weight (HW), and gastro-intestinal fat (GIF) differed in all the TRTS, with treatment 2 having the highest value of HW (28.3±2.55), and LW (44.2±1.60) was the highest on treatment 4 . The pH values in all TRTS were constant from Day1 to Day5, reached peak on Day6, and then declined on Day7. Meat from broilers given treatment 1 with MOLM (1000g/ton) had the highest lightness (L*) values. The redness (a*) values were the highest in meat from treatment 2 (750g/ton MOLM). Treatments had no effect on yellowness (b*) values and on drip loss of the breasts. During storage L* values were high from Day1 to Day5 and decreased from Day6 to Day7. Drip loss increased with storage time as expected. Treatment 4 (control) had the highest proportions of poly-unsaturated fatty acids (PUFA) (30.3±1.87). Treatment 1 (1000g/ton) had the highest proportion of saturated fatty acids (SFA) (60.9±1.87). Treatment 1 (1000g/ton) had the highest proportion of SFA (60.9±4.30). Treatment 2 (750g/ton) had the highest n-6/n-3 ratio than other TRTS. Days had no effect (P>0.05) on PUFA, SFA, and n-6/n-3 ratio. Treatment 1 had a highest amount of malondialdehyde (MDA), treatment 4 had no effect (P>0.05) on MDA . Storage time had an effect (P<0.05) on MDA levels, except for on Day1 and Day7. Day2 had the highest amount of MDA (0.7±0.08). The use of MOLM as an additive in broiler diets reduced lipid oxidation in meat, and maintained the quality of the broiler meat during storage. It also did not have any adverse effects on the growth performance of broilers. Therefore, it has the potential to be used as an additive in broiler diets.
- Full Text:
- Date Issued: 2012
- Authors: Wapi, Cwayita
- Date: 2012
- Subjects: Moringa , Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11822 , http://hdl.handle.net/10353/d1007546 , Moringa , Broilers (Chickens) , Meat -- Quality
- Description: Effects of feeding Moringa oleifera leaf meal as an additive on growth performance of chicken, physico- chemical shelf-life indicators, fatty acids profiles and lipid oxidation of broiler meat The main objective of the study was to determine the effect of M.oleifera leaf meal (MOLM) as an additive on growth performance, carcass characteristics, physico-chemical shelf-life indicators (colour, ultimate pH, driploss), fatty acids profiles and lipid oxidation of meat from broilers. A total of 432 1day old unsexed broiler chicks (Aviane 48) were randomly allocated to four dietary treatments (TRTS) in 72 cages. There were 18 cages per treatment and each cage allocated 6 chicks. Water and feed was provided at ad libitum. The feeding phases were, prestarter (0-7 Days), starter (8-18 Days), grower (19-28 Days), finisher (29-35 Days). The four TRTS contained graded levels of MOLM at 1000g/ton, 750g/ton, 500g/ton, and 0g/ton (control), respectively. The birds were slaughtered at 35 days of age. Breast muscles were sampled for meat, ultimate pH (pHu ), colour, drip loss over a 7 days shelf-life test. After each day’s test sub-samples were dipped in liquid nitrogen and kept at -180 C for thiobarbituric acid reactive substances determination. On Day1 and Day 7 extra sub-samples were also kept at -180 C for fatty acids analysis.The TRTS had no effect on average feed intake (AFI), feed conversion efficiency (FCE), and on average daily gain (ADG). Slaughter weight (SW), carcass weight (CW), dressing percentage and gizzard weight (GW) values were similar in all TRTS. Liver weight (LW), heart weight (HW), and gastro-intestinal fat (GIF) differed in all the TRTS, with treatment 2 having the highest value of HW (28.3±2.55), and LW (44.2±1.60) was the highest on treatment 4 . The pH values in all TRTS were constant from Day1 to Day5, reached peak on Day6, and then declined on Day7. Meat from broilers given treatment 1 with MOLM (1000g/ton) had the highest lightness (L*) values. The redness (a*) values were the highest in meat from treatment 2 (750g/ton MOLM). Treatments had no effect on yellowness (b*) values and on drip loss of the breasts. During storage L* values were high from Day1 to Day5 and decreased from Day6 to Day7. Drip loss increased with storage time as expected. Treatment 4 (control) had the highest proportions of poly-unsaturated fatty acids (PUFA) (30.3±1.87). Treatment 1 (1000g/ton) had the highest proportion of saturated fatty acids (SFA) (60.9±1.87). Treatment 1 (1000g/ton) had the highest proportion of SFA (60.9±4.30). Treatment 2 (750g/ton) had the highest n-6/n-3 ratio than other TRTS. Days had no effect (P>0.05) on PUFA, SFA, and n-6/n-3 ratio. Treatment 1 had a highest amount of malondialdehyde (MDA), treatment 4 had no effect (P>0.05) on MDA . Storage time had an effect (P<0.05) on MDA levels, except for on Day1 and Day7. Day2 had the highest amount of MDA (0.7±0.08). The use of MOLM as an additive in broiler diets reduced lipid oxidation in meat, and maintained the quality of the broiler meat during storage. It also did not have any adverse effects on the growth performance of broilers. Therefore, it has the potential to be used as an additive in broiler diets.
- Full Text:
- Date Issued: 2012
Medicinal properties of Moringa (Moringa Oleifera Lam) leaves and the effect of its use as a supplement on goat growth performance and meat characteristics
- Moyo, Busani https://orcid.org/0000-0001-7002-7266
- Authors: Moyo, Busani https://orcid.org/0000-0001-7002-7266
- Date: 2011-09
- Subjects: Moringa , Medicinal plants
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24376 , vital:62662
- Description: The main objective of the study was to determine if feeding goats with Moringa oleifera leaves would lead to an increase in productivity and in value of the meat. The proximate, van Soet, atomic absorption spectrophotometric and soxhlet extraction methods were used to determine the nutritional value M. oleifera leaves of the South African. The in-vitro antimicrobial screening methods were used to determine antimicrobial activities M. oleifera extracts while in vitro and invivo models were used to determine the antioxidant activities of M. oleifera leaves. An evaluation of the potential of M. oleifera leaf meal as a feed supplement in terms of its effect on helminth load, goat growth performance, carcass characteristics, meat quality attributes, nutritional and consumer sensory characteristics of goat meat was done. A total of 24, eight month old goats were randomly allocated to dietary treatments of M. oleifera leaf meal (MOL), sunflower seed cake (SC) and GH (grass hay) which was the control. All the groups were fed on basal diet of grass hay ad libitum and 200g wheat bran per head per day. The MOL group was given an additional 200 g of dried M. oleifera leaves while the SC group was offered 170 g sunflower seed cake per head/day. The study showed that the dried leaves had crude protein levels of 30.3 percent, polyunsaturated fatty acids (52.21 percent), Saturated fatty acids (43.31), n-3 (44.57 percent), n-6 (7.64 percent), 19 amino acids, vitamin E (77 mg/100 g) and Beta-carotene (18.5 mg/100 g). The M. oleifera leaf extracts showed antibacterial activities against Escherichia coli, Enterobacter cloace, Proteus vulgaris, Staphylococcus aureus and Micrococcus kristinae. The supplementation of goats with MOL and SC resulted in decreased feacal larval count and lower Haemonchus contortus, Trichostrongylus colubriforms and Oesophagastum columbianum worm burdens than those in the non-supplemented goats. Goats supplemented with SC and MOL had higher average daily weight gain and heavier carcasses than those in the GH group. Higher pH1 scores were observed in chevon from GH diet than the supplemented ones. The MOL and SC supplemented goats had chevon with higher values for lightness (L*) 24 hr post-mortem than the one from the GH group. The redness (a*) values of chevon 24 hr post mortem was highest in MOL supplemented goats. Warner Bratzler shear force (WBSF) values of SC (30.1 N) and MOL (29.8 N) supplemented goats were lower than those from GH diet (32.6 N). Chevon from goats fed GH diet had significantly higher cooking losses (29.5 percent) than that from MOL (25.4 percent) and SC (25.6 percent) fed groups. It was observed that chevon from MOL and SC supplemented groups had higher crude protein (23.57 and 22.95 percent, respectively) than the one from the GH group (21.20 percent). Cholesterol levels were higher in chevon from SC (42.84) supplemented goats than those from MOL (38.76) and GH (35.63 mg). Chevon from GH and MOL group had higher (P < 0.05) proportions of PUFA, n-3, PUFA/SFA ratio and lower n-6/n-3 ratio. Mean consumer scores for first bite, aroma, flavour and juiceness were higher in the MOL group than in the GH group (P < 0.05). The acetone extract exhibited higher concentrations of total flavonoids, flavonols, phenolics. The acetone extracts depicted higher percentage inhibition against DPPH, ABTS and nitric oxide radicals which were comparable with reference antioxidant (vitamin C and BHT). The M. oleifera leaf meal increased the antioxidant activity of GSH, SOD and catalase. Moringa oleifera leaves also exhibited medicinal properties by having anthelmintic, antibacterial activities and showed antioxidant properties. It was also observed that protein supplementation improved the animal growth performance, the physico-chemical characteristics, nutritional and fatty acids composition of meat hence meeting the consumer needs. , Thesis (PhD) -- Faculty of Science and Agriculture, 2011
- Full Text:
- Date Issued: 2011-09
- Authors: Moyo, Busani https://orcid.org/0000-0001-7002-7266
- Date: 2011-09
- Subjects: Moringa , Medicinal plants
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24376 , vital:62662
- Description: The main objective of the study was to determine if feeding goats with Moringa oleifera leaves would lead to an increase in productivity and in value of the meat. The proximate, van Soet, atomic absorption spectrophotometric and soxhlet extraction methods were used to determine the nutritional value M. oleifera leaves of the South African. The in-vitro antimicrobial screening methods were used to determine antimicrobial activities M. oleifera extracts while in vitro and invivo models were used to determine the antioxidant activities of M. oleifera leaves. An evaluation of the potential of M. oleifera leaf meal as a feed supplement in terms of its effect on helminth load, goat growth performance, carcass characteristics, meat quality attributes, nutritional and consumer sensory characteristics of goat meat was done. A total of 24, eight month old goats were randomly allocated to dietary treatments of M. oleifera leaf meal (MOL), sunflower seed cake (SC) and GH (grass hay) which was the control. All the groups were fed on basal diet of grass hay ad libitum and 200g wheat bran per head per day. The MOL group was given an additional 200 g of dried M. oleifera leaves while the SC group was offered 170 g sunflower seed cake per head/day. The study showed that the dried leaves had crude protein levels of 30.3 percent, polyunsaturated fatty acids (52.21 percent), Saturated fatty acids (43.31), n-3 (44.57 percent), n-6 (7.64 percent), 19 amino acids, vitamin E (77 mg/100 g) and Beta-carotene (18.5 mg/100 g). The M. oleifera leaf extracts showed antibacterial activities against Escherichia coli, Enterobacter cloace, Proteus vulgaris, Staphylococcus aureus and Micrococcus kristinae. The supplementation of goats with MOL and SC resulted in decreased feacal larval count and lower Haemonchus contortus, Trichostrongylus colubriforms and Oesophagastum columbianum worm burdens than those in the non-supplemented goats. Goats supplemented with SC and MOL had higher average daily weight gain and heavier carcasses than those in the GH group. Higher pH1 scores were observed in chevon from GH diet than the supplemented ones. The MOL and SC supplemented goats had chevon with higher values for lightness (L*) 24 hr post-mortem than the one from the GH group. The redness (a*) values of chevon 24 hr post mortem was highest in MOL supplemented goats. Warner Bratzler shear force (WBSF) values of SC (30.1 N) and MOL (29.8 N) supplemented goats were lower than those from GH diet (32.6 N). Chevon from goats fed GH diet had significantly higher cooking losses (29.5 percent) than that from MOL (25.4 percent) and SC (25.6 percent) fed groups. It was observed that chevon from MOL and SC supplemented groups had higher crude protein (23.57 and 22.95 percent, respectively) than the one from the GH group (21.20 percent). Cholesterol levels were higher in chevon from SC (42.84) supplemented goats than those from MOL (38.76) and GH (35.63 mg). Chevon from GH and MOL group had higher (P < 0.05) proportions of PUFA, n-3, PUFA/SFA ratio and lower n-6/n-3 ratio. Mean consumer scores for first bite, aroma, flavour and juiceness were higher in the MOL group than in the GH group (P < 0.05). The acetone extract exhibited higher concentrations of total flavonoids, flavonols, phenolics. The acetone extracts depicted higher percentage inhibition against DPPH, ABTS and nitric oxide radicals which were comparable with reference antioxidant (vitamin C and BHT). The M. oleifera leaf meal increased the antioxidant activity of GSH, SOD and catalase. Moringa oleifera leaves also exhibited medicinal properties by having anthelmintic, antibacterial activities and showed antioxidant properties. It was also observed that protein supplementation improved the animal growth performance, the physico-chemical characteristics, nutritional and fatty acids composition of meat hence meeting the consumer needs. , Thesis (PhD) -- Faculty of Science and Agriculture, 2011
- Full Text:
- Date Issued: 2011-09
Antioxidant activity and the quality of meat from goats and broilers supplemented with Moringa (Moringa Oleifera) leaves
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
- «
- ‹
- 1
- ›
- »