Towards understanding the effect of size variation on the aggressive and feeding behaviours of juvenile dusky kob Argyrosomus japonicus (Pisces: Sciaenidae)
- Authors: Babane, Siviwe Elvis
- Date: 2018
- Subjects: Argyrosomus -- Feeding and feeds , Aquaculture , Fishes -- Cannibalism , Fishes -- Feeding and feeds
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59592 , vital:27629
- Description: Many studies have been conducted on the effect of size-grading in other fish species. However, there is a paucity of scientific information on the effects of size variation on cannibalism of juvenile dusky kob. Thus, a study focusing on the effect of size variation on juvenile dusky kob aggressive and feeding (browsing) behaviours was conducted. Three separate groups of hatchery-reared juvenile dusky kob of were obtained from Oceanwise (Pty) Ltd for use in a series of three experimental trials. In all trials, juvenile fish of the same age were size-graded and the COV-value was used to determine the size variation. The focal fish (largest fish) was exposed to groups of fish with different size variation for 30-min. behavioural observations before and after feeding in randomised trials. The first experimental trial (Chapter 2) quantified the effect of increasing size variation and observation time on the aggressive and browsing behaviours of juvenile dusky kob. On average, juvenile dusky kob weighed 3.60 ± 0.68 g fish-1 and measured 5.8 ± 0.41 mm. Each focal fish was observed (a) before feeding in the morning, (b) 2 h after feeding, (c) 6 h after feeding and (d) 12 h after feeding. Fish increased browsing behaviours (averaging 6.60 ± 0.56) and decreased intimidating aggressive behaviours (18.60 ± 1.39) 12 h after feeding. Other aggressive behaviours occurred but did not differ between observation times. Aggressive and browsing behaviours positively correlated with size variation variables, predominantly, 12 h after feeding. An average frequency of 19 chases were observed positively correlated with size variation, followed by average frequencies of 17 body bites and browses, and 11 tail bites per 30 min. Some behaviours including average frequencies of 0.2 chases, 4 tail bites, 2.4 intimidating and 0.3 browsing behaviours negatively correlated with the size variation, generally closer to the last meal. These preliminary observations thus showed that fish have become hungry approximately 6-12 h after feeding and substituted certain behaviours for others as time after feeding passed and as size variation increased. The second experimental trial (Chapter 3) consisted of the observations further testing the relationship between aggressive behaviours and size variation of juveniles of dusky kob averaging 0.43 ± 0.27 g fish-1. The focal fish was exposed to groups of fish of four size variation (COV) treatments for observations before and 12 h after feeding. Aggressive behaviours positively correlated with size variation both before and 12 h after feeding. An average frequency of 437 body bites positively correlated more often with COV, followed by average frequencies of 365 intimidating behaviours and 199 tail bites per 30 min., respectively, before and 12 h after feeding. The least often exhibited aggressive behaviours averaged 26 chases while positively correlating with size variation on the times specified. An average frequency of 311 intimidating behaviours before and after feeding including average of 28 tail bites after feeding negatively correlated with size variation. This may relate to shift of behaviours depending on the needs and capacity of the fish. Apparently, aggressive fish can change its behaviour as a function of COV-values rather than the mean size of the other fish. The third trial (Chapter 4) investigated the effect of the aggressor’s (focal fish) size in relation its aggressive and browsing behaviours to other fish (non-focal fish). Juvenile fish used for this trial, on average, weighed 30 ± 7.63 g fish-1. Aggressive and browsing behaviours were observed in four treatments of a) high COV and mean weight below, b) low COV and mean weight less, c) high COV and mean weight equivalent to and d) low COV and mean weight higher than that of the focal fish. Increased frequencies of aggressive and browsing behaviours per 30 min. occurred in treatment A, sharing similar frequencies in treatment C, compared to the other treatments (B and D) which shared certain frequencies. The intimidating behaviours predominated, followed by browsing, body bites, chases and tail bites, respectively. The results of the overall study suggest that the time passed after feeding and increasing size variation and differences facilitated aggressive and browsing behaviours in juvenile dusky kob. Dusky kob showed increasing aggressive behaviours as early as in the first two weeks after hatching, averaging 0.43 ± 0.27 g fish-1 with the frequency correlating with size differences. Consistent size-grading technique in the same-age fish should be used to manage size variation associated with aggressive behaviours. The period of about 4-6 h after feeding may explain the noticeable increased aggressive and browsing behaviours. Thus, fish should be fed immediately before or after evacuation of their guts to maintain less-aggressive behaviours of juvenile dusky kob. Fish generally increased aggressive and browsing acts before and long time after feeding than closer to after feeding. The study has provided the fundamental scientific groundwork for fish farmers and future researchers can further explore size variation, time after feeding and gut evacuation rate as critical components of aggressive behaviours. The scientific knowledge of aggressive and cannibalistic behaviours has essential application in farming management to achieve improved survival and growth rates in juvenile fish.
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- Date Issued: 2018
Apparent digestibility coefficients of feed ingredients and essential amino acid requirements of dusky kob (Argyrosomus japonicus)
- Authors: Adesola, Abidemi Adejoke
- Date: 2017
- Subjects: Amino acids in animal nutrition , Fishes -- Feeding and feeds , Argyrosomus japonicus , Argyrosomus , Argyrosomus japonicus -- Nutrition , Argyrosomus -- Nutrition , Argyrosomus japonicus -- Feeding and feeds , Argyrosomus -- Feeding and feeds
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/32140 , vital:24014
- Description: Important to the evaluation of potential feed ingredients for inclusion in fish diets should be their digestibility and amino acid requirement so that feeds can be formulated on a digestible basis rather than a gross nutrient basis. This thesis established techniques for faecal collection, the validity of digestibility markers, it determined apparent digestibility coefficients for various novel feed ingredients, and it established the optimal lysine requirement for Argyrosomus japonicus, which was used to estimate each of the essential amino acid requirements by using the ideal protein concept. There were no significant differences in dry matter apparent digestibility coefficients when faeces were collected by stripping (77.0 %), dissection (80.1 %) or settlement (83.5 %). Faecal collection by the settlement method produced the most reliable digestibility data. Apparent digestibility coefficients for dry matter, crude protein and energy calculated using acid-insoluble ash were higher (84.0 %, 93.2 % and 93.0 %, respectively) than those using chromic oxide (55.7 %, 79.1 % and 78.2 %) and titanium dioxide (58.6 %, 79.7 % and 80.5 %). The magnitude of variation in digestibility coefficients obtained using acid-insoluble ash was always lower than that obtained with the other markers. Therefore, acid-insoluble ash was preferred as a dietary marker. Using the above protocol, protein and amino acid digestibility of some animal and plant protein ingredients were evaluated in a series of experiments. The first trial compared the apparent digestibility coefficients of some animal products included as single protein source in the test diets. Apparent protein digestibility values were 84.5 %, 83.8 %, 85.8 % and 83.1 % for fishmeal-prime, fishmeal-standard, poultry meal and pork meal, respectively. Apparent digestibility coefficients for poultry meal were comparable to those of fishmeal, which indicate its potential as a substitute for fishmeal in the diets of A. japonicus. The second trial determined the apparent coefficients of plant and animal protein sources included at 30 % into a practical reference diet (70 %). Apparent protein digestibility ranged from 92.4 % in sunflower meal to 85.5 % in corn gluten meal. Soybean meal is a promising feed ingredient in A. japonicus due to the high apparent digestibility of its protein (92.0 %) and essential amino acid digestibility (mean average 91.4 %). A fundamental assumption in fish feed formulation is that the digestibility of nutrients is additive, i.e., digestibility of a nutrient in one ingredient does not interact with the digestibility of the same nutrient in another ingredient. In the third trial, additivity of feed ingredients was tested using pork meal and poultry meal. The results indicate that the apparent digestibility coefficient of animal protein ingredients could be calculated from compound diets to accurately determine protein and amino acid digestibility in A. japonicus, and possibly other carnivorous fish species. Dietary essential amino acid requirements were determined for juvenile A. japonicus in two trials. A dose-response study was conducted using crystalline lysine to determine the optimal requirement of dietary lysine for A. japonicus. Optimal dietary lysine was estimated at 31.7 g kg-¹ dry diet, corresponding to 73.5 g kg-¹ of dietary protein, based on specific growth rate and broken-line segmented regression analyses. Dietary requirements for other essential amino acids ranged from 22 g kg-¹ (histidine) to 71 g kg-¹ (leucine) crude protein. The results of the present study provided a research tool that could be used to assess and verify the conclusions of earlier dietary work on A. japonicus and in further studies to develop least cost diet formulations for this species. The study also adds to the knowledge of the nutritional requirements of A. japonicus by providing information on the digestibility of plants and animal protein ingredients. It also contributes to future dietary research for this species because this study determines, for the first time, the most suitable methods for investigating the digestibility of raw materials for A. japonicus.
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- Date Issued: 2017
The effect of carbohydrate on dusky kob, Argyrosomus japonicus, fed pelleted diets
- Authors: Mabasa, Nyiko Charity
- Date: 2017
- Subjects: Carbohydrates in animal nutrition , Fishes -- Feeding and feeds , Argyrosomus japonicus , Argyrosomus , Argyrosomus japonicus -- Nutrition , Argyrosomus -- Nutrition , Argyrosomus japonicus -- Feeding and feeds , Argyrosomus -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/44895 , vital:25450
- Description: The dusky kob, Argyrosomus japonicus, is an emerging commercial marine aquaculture species in South Africa. Attributes such as market acceptance, fast growth rates, tolerance to sibling density, acceptance of pelleted feed and adaptability to intensive aquaculture conditions make it a good candidate. Feed, the largest running cost in most aquaculture operations, is a limiting factor in dusky kob production as its nutritional requirements are not well understood. The present project formed part of a research program to develop a locally produced, least cost and sustainable feed that will support the growth and health of dusky kob. The approximate protein and energy requirements for kob fed formulated feeds have been established in earlier research, but the ability of kob to utilize carbohydrates as an energy source has not previously been investigated. The aim of this study was to determine the efficiency of dusky kob to utilize graded levels of carbohydrate (pregelatinized maize starch - PGMS) in pelleted diets. The research objectives were to determine the effect of dietary carbohydrate levels on:i) growth and feed utilization; ii) post prandial blood glucose levels; and iii) general fish health, gut bacterial composition and liver glycogen content of juvenile dusky kob. Hatchery reared juvenile dusky kob (5 g) were acclimatized in a recirculating experimental aquaculture system at the Department of Ichthyology and Fisheries Science at Rhodes University. They were fed trout crumble starter feed for three weeks before the start of the feeding experiment. Five diets containing 4.1, 8.2, 16.4 and 24.6% carbohydrate, hereafter referred to as 4.1C, 8.2C, 16.4C and 24.6C respectively, were formulated with pregelatinized maize starch as a carbohydrate source and fishmeal as the main protein source. Fish were fed these diets twice daily at 3.85% body weight per day for three months. Specific growth rates and feed conversion rations differed significantly between the four dietary treatments. Growth rate increased with increasing carbohydrate up to 16.4%, after which a significant decline was observed. Diet 16.4C produced the highest specific growth rate (SGR) of 1.84 ± 0.05% body weight/day, and the lowest feed conversion ratio of 1.28 ± 0.08. While the lowest specific growth rate and highest feed conversion ratio of 0.79 ± 0.05 and 2.14 ± 0.13 respectively, were found for diet 24.6C. A third order polynomial regression, using SGR, determined the optimum carbohydrate inclusion for dusky kob to be 16.72%. The general structure of the liver was similar between all fish fed the dietary treatments and all fish liver samples displayed a certain degree of lipid vacuolization of the hepatocytes. Evidence of starvation was observed in the livers of fish fed diet 24.6C. However, no differences in the amount of liver glycogen were observed. Gut bacterial composition did not differ among the different diets or between the different sections of the gut. Differences were however observed in the diversity of the bacterial community structure at the start (when they were fed commercial trout feed) and end of the experimental period (after being fed a fishmeal-maize diet). The rate at which glucose was cleared from the blood differed significantly between diets. The low carbohydrate diets (i.e. diet 4.1C and 8.2C) showed a steady rate of glucose removal from the blood over a 48 h experimental period. Sharp increases in blood glucose concentration were observed in diet 16.4C and diet 24.6C, with highest glucose concentrations of 7.18 ± 1.81 and 8.05 ± 2.35 mmol/l respectively, observed 24 h after feeding. The blood glucose concentration of the fish fed diet 16.4C however returned to resting glucose concentration after 48 h, while that of the fish fed diet 24.6C did not. The results demonstrated that the level of dietary carbohydrate inclusion has a significant effect on the growth and health of the fish. The optimum carbohydrate inclusion in dusky kob diets is indicated to be 16.7%, which gave the best growth rate and maximum protein sparing effect without adverse effects on fish health. These findings are important for diet formulation and producing ‘least-cost’ diets for dusky kob farming.
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- Date Issued: 2017
The effect of dietary fish oil replacement with soybean oil on growth and health of dusky kob, Argyrosomus japonicus (Pisces: Sciaenidae)
- Authors: Rossetti, Nani Adami
- Date: 2012
- Subjects: Sciaenidae , Fish culture , Argyrosomus -- Growth , Argyrosomus -- Feeding and feeds , Argyrosomus -- Health , Fish oils as feed , Soy oil , Lipids , Eicosapentaenoic acid , Docosahexaenoic acid
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5272 , http://hdl.handle.net/10962/d1005116 , Sciaenidae , Fish culture , Argyrosomus -- Growth , Argyrosomus -- Feeding and feeds , Argyrosomus -- Health , Fish oils as feed , Soy oil , Lipids , Eicosapentaenoic acid , Docosahexaenoic acid
- Description: Lipids are essential components for fish because they contain fatty acids that are vital for regular growth and health. Fish oil is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are essential fatty acids for carnivorous fish, and therefore this product has traditionally been used as the main source of lipids in fish feeds. However, with declining fisheries resources worldwide and the rapid expansion of the aquaculture industry pressuring this finite resource, such ingredients are becoming less available and more expensive. It is therefore necessary to explore the utilization of ingredients that are sustainable and competitive alternatives to fish oil in marine finfish feeds. This work investigated the effects of the substitution of fish oil with soybean oil on the growth performance, feed efficiency, fatty acid composition of the liver tissue and some health parameters in juvenile dusky kob, Argyrosomus japonicus; an increasingly popular sciaenid marine aquaculture species in South Africa. Six diets (18 % total lipid and 46 % protein) with increasing percentage substitution of fish oil with soybean oil (1, 14, 28, 42, 56 and 70 %) were fed to juvenile kob. After 84 days of feeding these diets to the fish, no significant differences in fish length and weight between treatments were observed. However, there was a significant trend of a decrease in specific growth rate, ranging from (± standard error) 0.87 ± 0.06 to 0.72 ± 0.04 % body weight day⁻¹, and condition factor, ranging from 1.59 ± 0.03 to 1.54 ± 0.02, with increasing vegetable oil replacement in the diets between days 56 and 84. There were no differences in red blood cell count, haematocrit and haemoglobin concentration after 206 days of feeding. However, visceral fat index (VFI) increased significantly from 1.08 ± 0.17 % for fish fed diets with 28 % soybean oil, to 2.24 ± 0.15 % for fish fed diets with 70 % soybean oil. Similarly, hepatosomatic index (HSI) increased significantly from 0.84 ± 0.08 % to 1.80 ± 0.12 % in the control diet and the 56 % soybean oil diet, respectively. After 206 days of feeding, fish fed diets with 42 to 70 % soybean oil showed greater number of lipid vacuoles in the liver, which were also larger in size, and hepatocytes nuclei were displaced to the cell periphery. The fatty acid composition of the liver tissue strongly corresponded to the fatty acid composition of the diets. Linoleic acid accumulated in the liver of the fish fed increasing soybean oil in the diets. In contrast, EPA and DHA decreased from 13.63 to 1.97 %, and 14.34 to 3.28 %, respectively, in the liver tissue of fish fed diets with increasing soybean oil content; consequently the n-3/n-6 ratio was also significantly reduced with inclusion of vegetable oil in the diets. The trend of decreasing growth rate with increasing oil replacement towards the end of the trial corresponds with increases in VFI, HSI, as well as the fatty acid accumulation and lipid vacuoles in the liver. This suggests that dusky kob is less able to metabolise soybean oil at increased substitution levels which would account for the poorer growth at higher levels. The dependence of fish on dietary marine oil decreased significantly with each inclusion of soybean oil in the diets. Nonetheless, the calculations based on the nutrient ratio presented positive outcomes for all treatments, that is, values of marine oil dependency ratio were below one for all treatments. It is concluded that soybean oil can replace fish oil in formulated diets for dusky kob up to a level of 28 % of total dietary lipids, as evidenced by the good growth and feed efficiency, and no apparent negative health effects observed up to this level.
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- Date Issued: 2012