Behavior of palladium nanoparticles in the absence or presence of cobalt tetraaminophthalocyanine for the electrooxidation of hydrazine
- Maringa, Audacity, Nyokong, Tebello
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189796 , vital:44932 , xlink:href="https://doi.org/10.1002/elan.201400028"
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE.
- Full Text:
- Date Issued: 2014
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189796 , vital:44932 , xlink:href="https://doi.org/10.1002/elan.201400028"
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE.
- Full Text:
- Date Issued: 2014
Behavior of Palladium Nanoparticles in the Absence or Presence of Cobalt Tetraaminophthalocyanine for the Electrooxidation of Hydrazine
- Maringa, Audacity, Nyokong, Tebello
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Language: English
- Type: Article
- Identifier: vital:7305 , http://hdl.handle.net/10962/d1020382
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE. , Original publication is available at http://dx.doi.org/10.1002/elan.201400028
- Full Text: false
- Date Issued: 2014
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Language: English
- Type: Article
- Identifier: vital:7305 , http://hdl.handle.net/10962/d1020382
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE. , Original publication is available at http://dx.doi.org/10.1002/elan.201400028
- Full Text: false
- Date Issued: 2014
Behaviour-based health and safety management in construction: a leadership-focused approach
- Authors: Okorie, Victor Nnannaya
- Date: 2014
- Subjects: Construction industry -- Safety measures , Construction industry -- Accidents -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9676 , http://hdl.handle.net/10948/d1021034
- Description: The construction industry remains a pillar in the South African economy, generating employment and wealth. Nonetheless, the industry is reportedly bedevilled by a high rate of accidents and serious injuries that often lead to permanent deformation and fatalities among workers and the general public. These accidents and injuries manifest due to poor construction health and safety (H&S) performance that is often related to poor H&S leadership of the key project leaders involved in the construction business. Shortcomings pertain to client leadership in terms of involvement and commitment to H&S and project H&S related decisions of professionals (designers, project managers, quantity surveyors and engineers). They also pertain to contractor related aspects such as H&S management systems and leadership at all levels of management. In addition, contractors’ inadequacies of H&S management relative to workplace planning and materials related issues have continued to marginalise H&S performance in construction. The H&S leadership of key project leaders is very important in creating a culture of H&S in the workplace. This study examined the H&S management practices and leadership of the key project leaders that contribute to at-risk work practices or unsafe behaviour of workers. Presently, there is limited or no research in South African construction on how the H&S leadership of the key project leaders contributes to at-risk work practices or unsafe behaviour of workers. The methodology employed in the study included an extensive review of relevant literature, which enabled the field work to proceed unhindered. The quantitative survey and qualitative inquiry was conducted with the key construction participants in South African construction. Focus group discussions and interviews were used in a complementary manner. Accordingly, the sampling technique for the study comprised simple random and purposive sampling. The study revealed apparent poor leadership and lack of involvement and commitment to workers’ H&S by the key project leaders. Notably, there is statistical evidence of poor H&S management practices among contractors in terms of top management commitment to and involvement in project H&S. This statistical evidence includes poor H&S education and training of workers and lack of workers’ involvement and participation in H&S matters, which is the tenet of behaviour-based H&S anagement. The model of leadership influence on worker H&S behaviour developed in this research constitutes an innovative contribution to construction H&S performance improvement through a leadership-focused approach. The study has established a basic level of awareness and understanding among key project leaders in that their upstream decisions during the project planning and construction phases have significant influence on workers’ safe or unsafe behaviour or at-risk work practices. The study strongly advocates transparent leadership, ethical behaviour among clients in public and private sectors, designers’ critical H&S decisions, project managers’ and quantity surveyors’ commitment towards project H&S and contractors’ adoption and implementation of behaviour-based H&S management systems. The developed leadership influence model of worker H&S behaviour in the study provides a useful guide for the key project leaders to realise the desired H&S performance improvement in the South African construction industry.
- Full Text:
- Date Issued: 2014
- Authors: Okorie, Victor Nnannaya
- Date: 2014
- Subjects: Construction industry -- Safety measures , Construction industry -- Accidents -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9676 , http://hdl.handle.net/10948/d1021034
- Description: The construction industry remains a pillar in the South African economy, generating employment and wealth. Nonetheless, the industry is reportedly bedevilled by a high rate of accidents and serious injuries that often lead to permanent deformation and fatalities among workers and the general public. These accidents and injuries manifest due to poor construction health and safety (H&S) performance that is often related to poor H&S leadership of the key project leaders involved in the construction business. Shortcomings pertain to client leadership in terms of involvement and commitment to H&S and project H&S related decisions of professionals (designers, project managers, quantity surveyors and engineers). They also pertain to contractor related aspects such as H&S management systems and leadership at all levels of management. In addition, contractors’ inadequacies of H&S management relative to workplace planning and materials related issues have continued to marginalise H&S performance in construction. The H&S leadership of key project leaders is very important in creating a culture of H&S in the workplace. This study examined the H&S management practices and leadership of the key project leaders that contribute to at-risk work practices or unsafe behaviour of workers. Presently, there is limited or no research in South African construction on how the H&S leadership of the key project leaders contributes to at-risk work practices or unsafe behaviour of workers. The methodology employed in the study included an extensive review of relevant literature, which enabled the field work to proceed unhindered. The quantitative survey and qualitative inquiry was conducted with the key construction participants in South African construction. Focus group discussions and interviews were used in a complementary manner. Accordingly, the sampling technique for the study comprised simple random and purposive sampling. The study revealed apparent poor leadership and lack of involvement and commitment to workers’ H&S by the key project leaders. Notably, there is statistical evidence of poor H&S management practices among contractors in terms of top management commitment to and involvement in project H&S. This statistical evidence includes poor H&S education and training of workers and lack of workers’ involvement and participation in H&S matters, which is the tenet of behaviour-based H&S anagement. The model of leadership influence on worker H&S behaviour developed in this research constitutes an innovative contribution to construction H&S performance improvement through a leadership-focused approach. The study has established a basic level of awareness and understanding among key project leaders in that their upstream decisions during the project planning and construction phases have significant influence on workers’ safe or unsafe behaviour or at-risk work practices. The study strongly advocates transparent leadership, ethical behaviour among clients in public and private sectors, designers’ critical H&S decisions, project managers’ and quantity surveyors’ commitment towards project H&S and contractors’ adoption and implementation of behaviour-based H&S management systems. The developed leadership influence model of worker H&S behaviour in the study provides a useful guide for the key project leaders to realise the desired H&S performance improvement in the South African construction industry.
- Full Text:
- Date Issued: 2014
Bells as memorials in South Africa to the Great (1914-18) War
- Lewis, Colin A, De Wet, Tertius, Teugels, Jet L, Van Deventer, Pieta J U
- Authors: Lewis, Colin A , De Wet, Tertius , Teugels, Jet L , Van Deventer, Pieta J U
- Date: 2014
- Language: English
- Type: Article , text
- Identifier: vital:6192 , http://hdl.handle.net/10962/d1013418
- Description: The contribution of South Africa to the allied cause in the Great War, and the sacrifice of so many lives of the White and Coloured populations, is memorialised on bells of the Cape Town carillon, on ringing, and on clock and other bells. The contribution of the Black population awaits recognition. Restoration of the Cape Town carillon so that it can again be played effectively, would be a fitting memorial to those who lost their lives in the non-combative roles that were open to the majority population of South Africa. Completion of the ring at what is now Queenstown cathedral would also be a fitting tribute to the bravery and unstinting service of so many South Africans during the Great War. , Colin Lewis was Professor of Geography at Rhodes University, Grahamstown, South Africa from 1989 until his retirement at the end of 2007. In 1990, with the strong support of the incumbent Vice-Chancellor, Dr Derek Henderson, he instigated the Certificate in Change Ringing (Church Bell Ringing) in the Rhodes University Department of Music and Musicology - the first such course to be offered in Africa. Since that date he has lectured in the basic theory, and taught the practice of change ringing. He is the Ringing Master of the Cathedral of St Michael and St George, Grahamstown, South Africa.
- Full Text:
- Date Issued: 2014
- Authors: Lewis, Colin A , De Wet, Tertius , Teugels, Jet L , Van Deventer, Pieta J U
- Date: 2014
- Language: English
- Type: Article , text
- Identifier: vital:6192 , http://hdl.handle.net/10962/d1013418
- Description: The contribution of South Africa to the allied cause in the Great War, and the sacrifice of so many lives of the White and Coloured populations, is memorialised on bells of the Cape Town carillon, on ringing, and on clock and other bells. The contribution of the Black population awaits recognition. Restoration of the Cape Town carillon so that it can again be played effectively, would be a fitting memorial to those who lost their lives in the non-combative roles that were open to the majority population of South Africa. Completion of the ring at what is now Queenstown cathedral would also be a fitting tribute to the bravery and unstinting service of so many South Africans during the Great War. , Colin Lewis was Professor of Geography at Rhodes University, Grahamstown, South Africa from 1989 until his retirement at the end of 2007. In 1990, with the strong support of the incumbent Vice-Chancellor, Dr Derek Henderson, he instigated the Certificate in Change Ringing (Church Bell Ringing) in the Rhodes University Department of Music and Musicology - the first such course to be offered in Africa. Since that date he has lectured in the basic theory, and taught the practice of change ringing. He is the Ringing Master of the Cathedral of St Michael and St George, Grahamstown, South Africa.
- Full Text:
- Date Issued: 2014
Best practice guideline for the transition of final year nursing students to professional nurses in the military health service in South Africa
- Authors: Caka, Ernestina Masebina
- Date: 2014
- Subjects: School-to-work transition -- South Africa , Nurses -- Training of -- South Africa , Nursing students -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10070 , http://hdl.handle.net/10948/d1021097
- Description: The transition period from student nurses to professional nurses has been acknowledged as being very stressful, particularly in the military health service due to the dual transition. The phase is marked as final year nursing students try to consolidate the experience and knowledge gained during their four year training period with clinical decision making and problem solving skills being applied in the work environment. The students require support and guidance to effect a successful transition from being a student to being a professional nurse. The transition of students in the military health setting might be experienced differently due to the context that is vastly different from the other health care settings (Moore, 2006:541). The aim of the research was to explore the experiences of role transition of final year nursing students, particularly their preparedness to take up the role of a professional nurse in the military health setting in order to assist managers and educators to support and facilitate this professional adjustment appropriately. A qualitative, descriptive, contextual design was employed for the study and followed a three-phase approach. Phase one comprised a qualitative approach, where semi-structured and focus group interviews were conducted to gather the data. Nurse managers, nurse educators, final year nursing students and novice professional nurses formed part of the population for the study. Creswell’s method of data analysis was employed in analysing the data. The second phase dealt with the integrative review of literature on the transition of final year nursing students into professional nurses. Data extracted from the guidelines formed themes that were triangulated to form phase three of the study. Lastly, a best practice guideline was developed to facilitate the transition period of final year nursing students to professional nurses. Principles of trustworthiness were adhered to, participants were treated in a fair manner and confidential information was not divulged without the consent of the participants. Participants were asked to take part voluntarily and without coercion. Ethical approval was requested to give consent for the study to be undertaken and ethical principles were adhered to throughout the study. Findings were then disseminated after the conclusion of the study.
- Full Text:
- Date Issued: 2014
- Authors: Caka, Ernestina Masebina
- Date: 2014
- Subjects: School-to-work transition -- South Africa , Nurses -- Training of -- South Africa , Nursing students -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10070 , http://hdl.handle.net/10948/d1021097
- Description: The transition period from student nurses to professional nurses has been acknowledged as being very stressful, particularly in the military health service due to the dual transition. The phase is marked as final year nursing students try to consolidate the experience and knowledge gained during their four year training period with clinical decision making and problem solving skills being applied in the work environment. The students require support and guidance to effect a successful transition from being a student to being a professional nurse. The transition of students in the military health setting might be experienced differently due to the context that is vastly different from the other health care settings (Moore, 2006:541). The aim of the research was to explore the experiences of role transition of final year nursing students, particularly their preparedness to take up the role of a professional nurse in the military health setting in order to assist managers and educators to support and facilitate this professional adjustment appropriately. A qualitative, descriptive, contextual design was employed for the study and followed a three-phase approach. Phase one comprised a qualitative approach, where semi-structured and focus group interviews were conducted to gather the data. Nurse managers, nurse educators, final year nursing students and novice professional nurses formed part of the population for the study. Creswell’s method of data analysis was employed in analysing the data. The second phase dealt with the integrative review of literature on the transition of final year nursing students into professional nurses. Data extracted from the guidelines formed themes that were triangulated to form phase three of the study. Lastly, a best practice guideline was developed to facilitate the transition period of final year nursing students to professional nurses. Principles of trustworthiness were adhered to, participants were treated in a fair manner and confidential information was not divulged without the consent of the participants. Participants were asked to take part voluntarily and without coercion. Ethical approval was requested to give consent for the study to be undertaken and ethical principles were adhered to throughout the study. Findings were then disseminated after the conclusion of the study.
- Full Text:
- Date Issued: 2014
Beyond black and white: black solidarity in post-apartheid South Africa
- Authors: Majavu, Phumlani
- Date: 2014
- Subjects: Black nationalism -- South Africa , Race relations -- South Africa , South Africa -- Politics and government -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2887 , http://hdl.handle.net/10962/d1016359
- Description: Almost 20 years after the white Nationalist government was voted out, some black South Africans believe that black solidarity is still necessary in South Africa. These people argue that since post-apartheid South Africa is still marred with racial injustice, it makes sense for blacks to advocate for black solidarity. Although it is true that black solidarity played an important role in the struggle against apartheid, in this thesis I argue that the struggle against current forms of racial injustice does not necessarily require black solidarity. This is not to deny the prevailing racialized oppression in the post-apartheid era, nor to deny the importance of black solidarity in the past; rather the point I am making is that the current form of racial oppression is somewhat different from the one before 1994. Hence I argue in this thesis that the current form of racial oppression requires us to do certain things differently. Doing things differently means improving upon the strategies of the past. For this to happen, I argue that every human being who believes in and is committed to racial justice ought to be included in the struggle for justice. Change, after all, is brought about when committed human beings work together for liberation and justice.
- Full Text:
- Date Issued: 2014
- Authors: Majavu, Phumlani
- Date: 2014
- Subjects: Black nationalism -- South Africa , Race relations -- South Africa , South Africa -- Politics and government -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2887 , http://hdl.handle.net/10962/d1016359
- Description: Almost 20 years after the white Nationalist government was voted out, some black South Africans believe that black solidarity is still necessary in South Africa. These people argue that since post-apartheid South Africa is still marred with racial injustice, it makes sense for blacks to advocate for black solidarity. Although it is true that black solidarity played an important role in the struggle against apartheid, in this thesis I argue that the struggle against current forms of racial injustice does not necessarily require black solidarity. This is not to deny the prevailing racialized oppression in the post-apartheid era, nor to deny the importance of black solidarity in the past; rather the point I am making is that the current form of racial oppression is somewhat different from the one before 1994. Hence I argue in this thesis that the current form of racial oppression requires us to do certain things differently. Doing things differently means improving upon the strategies of the past. For this to happen, I argue that every human being who believes in and is committed to racial justice ought to be included in the struggle for justice. Change, after all, is brought about when committed human beings work together for liberation and justice.
- Full Text:
- Date Issued: 2014
Beyond coming out: lesbians’ (alternative) stories of sexual identity told in post-Apartheid South Africa
- Gibson, Alexandra F, Macleod, Catriona I
- Authors: Gibson, Alexandra F , Macleod, Catriona I
- Date: 2014
- Language: English
- Type: Book chapter
- Identifier: vital:6304 , http://hdl.handle.net/10962/d1016063
- Description: Over the last several decades, the ‘coming out’i story has become entrenched as the central narrative with which lesbian, gay and bisexual (LGB) people can narrate their experiences of claiming a sexual identity and storying their lives in general (Bacon, 1998; Blackburn, 2009). It has developed into a “canonical narrative” (Bruner, 1987, p. 15), or a culturally recognisable story for LGB people, in that it involves the recounting of a series of familiar events in moving from a place of shame to one of self-acceptance about one’s sexual identity (Cohler & Hammack, 2007; Plummer, 1995). The ‘coming out’ canonical narrative additionally operates as a counter-narrative, which has enabled LGB people to voice their sexuality within heterosexist and heteronormative confines (Blackburn, 2009). Nevertheless, there are limitations (and limiting effects) to this narrative, and further refinement of how we understand sexual identity narratives is required. To illustrate this argument, we draw on a narrative-discursive study of eight lesbians’ stories of sexual identity in post-apartheid South Africa.
- Full Text:
- Date Issued: 2014
- Authors: Gibson, Alexandra F , Macleod, Catriona I
- Date: 2014
- Language: English
- Type: Book chapter
- Identifier: vital:6304 , http://hdl.handle.net/10962/d1016063
- Description: Over the last several decades, the ‘coming out’i story has become entrenched as the central narrative with which lesbian, gay and bisexual (LGB) people can narrate their experiences of claiming a sexual identity and storying their lives in general (Bacon, 1998; Blackburn, 2009). It has developed into a “canonical narrative” (Bruner, 1987, p. 15), or a culturally recognisable story for LGB people, in that it involves the recounting of a series of familiar events in moving from a place of shame to one of self-acceptance about one’s sexual identity (Cohler & Hammack, 2007; Plummer, 1995). The ‘coming out’ canonical narrative additionally operates as a counter-narrative, which has enabled LGB people to voice their sexuality within heterosexist and heteronormative confines (Blackburn, 2009). Nevertheless, there are limitations (and limiting effects) to this narrative, and further refinement of how we understand sexual identity narratives is required. To illustrate this argument, we draw on a narrative-discursive study of eight lesbians’ stories of sexual identity in post-apartheid South Africa.
- Full Text:
- Date Issued: 2014
Biochemical mechanisms towards understanding Alzheimer's disease
- Authors: Padayachee, Eden Rebecca
- Date: 2014
- Subjects: Alzheimer's disease Nitric-oxide synthase Biochemical markers Amyloid beta-protein Peptide hormones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4103 , http://hdl.handle.net/10962/d1011092
- Description: The start of the amyloidogenic pathway in Alzheimer’s disease (AD) begins with the deposition of the Aβ₁₋₄₂ peptide surrounded by astrocytes. High levels of arginine and low amounts of neuronal nitric oxide synthase (nNOS) are associated with AD. These astrocytes store reserve arginine that is eventually metabolized by nNOS, within the vicinity of the Aβ₁₋₄₂ peptide. We propose the existence of an association vs. dissociation equilibrium between Aβ and nNOS such that nNOS is an amyloidogenic catalyst for fibrils. When Aβ binds to nNOS, it inhibits the activity of the enzyme (association phase). However when the amyloid peptide dissociates into a form that can no longer bind, later deduced as a fibril, the activity is restored. Thus, the interaction of Aβ with nNOS could serve to regulate the interaction between nNOS and arginine by restoring activity of the enzyme but at the same time promoting fibrillogenesis. Given this event occurring with the neuron, both nNOS and amyloid can serve as a biomarker for the early onset of AD. The enzyme nNOS catalyzed the formation of fibrils in the presence of Aβ peptides, while Ag nps were shown to reverse the fibril formation from Aβ peptides more so than Au and curcumin either through electrostatic or π-π stacking (aromatic) influences. Our studies have shown that the fragments of Aβ₁₋₄₂ i.e. the pentapeptide (Aβ₁₇₋₂₁) and the three glycine zipper peptides (Aβ₂₅₋₂₉, Aβ₂₉₋₃₃, Aβ₃₃₋₃₇) and the full length glycine zipper stretch (Aβ₂₅₋₃₇) all inhibited nNOS activity to varying degrees. The peptides Aβ₁₇₋₂₁ and Aβ₂₉₋₃₃ with their respective Ki values of 5.1 μM and 7.5 μM inhibited the enzyme the most. The Ki values for reversed sequenced peptides (Aβ₁₇₋₂₁r and Aβ₂₉₋₃₃r) were two fold greater than that of the original peptides while the Ki values for the polar forms (Aβ₁₇₋₂₁p and Aβ₂₉₋₃₃p) were between 3-4 fold greater than that of the original peptides. It was also found that Ag nps (Ki = 0.12 μM) inhibited the activity of nNOS the most compared to Au nps; (Ki = 0.15 μM) and curcumin (Ki = 0.25 μM). At 298K, all the ligands bound at a single site on the enzyme (n=1) and a single Trp residue (θ =1), (later identified as Trp678) was made available on the enzyme surface for quenching by the ligands. Increasing the temperature from 298K-313K, increased the value of Ksv and pointed to a dynamic quenching mechanism for Aβ peptides, nps and curcumin interaction with nNOS. The positive signs for entropy and enthalpy for all Aβ peptides nps and curcumin pointed to hydrophobic–hydrophobic interaction with the enzyme. The fact that Kd increased with temperature emphasized the endothermic nature of the binding reaction and the requirement of thermal energy to aid in diffusion of the ligand to the active site. It was concluded that the binding reaction between the ligands and nNOS was non-spontaneous and endothermic at low temperatures (+ΔG) but spontaneous at high temperatures (-ΔG). The two amino acids Tyr706 and Trp678 moved from their original positions, subject to ligand binding. Trp678 moved a minimum distance of 5 Å toward the heme while Tyr706 moved a maximum distance of 14 Å away from the heme. AutoDock 4.2 was a valuable tool in monitoring the distance of Trp678 within the enzyme interior and fluorescence resonance energy transfer (FRET) was efficient in monitoring the distance moved by Trp residues on the enzyme surface.
- Full Text:
- Date Issued: 2014
- Authors: Padayachee, Eden Rebecca
- Date: 2014
- Subjects: Alzheimer's disease Nitric-oxide synthase Biochemical markers Amyloid beta-protein Peptide hormones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4103 , http://hdl.handle.net/10962/d1011092
- Description: The start of the amyloidogenic pathway in Alzheimer’s disease (AD) begins with the deposition of the Aβ₁₋₄₂ peptide surrounded by astrocytes. High levels of arginine and low amounts of neuronal nitric oxide synthase (nNOS) are associated with AD. These astrocytes store reserve arginine that is eventually metabolized by nNOS, within the vicinity of the Aβ₁₋₄₂ peptide. We propose the existence of an association vs. dissociation equilibrium between Aβ and nNOS such that nNOS is an amyloidogenic catalyst for fibrils. When Aβ binds to nNOS, it inhibits the activity of the enzyme (association phase). However when the amyloid peptide dissociates into a form that can no longer bind, later deduced as a fibril, the activity is restored. Thus, the interaction of Aβ with nNOS could serve to regulate the interaction between nNOS and arginine by restoring activity of the enzyme but at the same time promoting fibrillogenesis. Given this event occurring with the neuron, both nNOS and amyloid can serve as a biomarker for the early onset of AD. The enzyme nNOS catalyzed the formation of fibrils in the presence of Aβ peptides, while Ag nps were shown to reverse the fibril formation from Aβ peptides more so than Au and curcumin either through electrostatic or π-π stacking (aromatic) influences. Our studies have shown that the fragments of Aβ₁₋₄₂ i.e. the pentapeptide (Aβ₁₇₋₂₁) and the three glycine zipper peptides (Aβ₂₅₋₂₉, Aβ₂₉₋₃₃, Aβ₃₃₋₃₇) and the full length glycine zipper stretch (Aβ₂₅₋₃₇) all inhibited nNOS activity to varying degrees. The peptides Aβ₁₇₋₂₁ and Aβ₂₉₋₃₃ with their respective Ki values of 5.1 μM and 7.5 μM inhibited the enzyme the most. The Ki values for reversed sequenced peptides (Aβ₁₇₋₂₁r and Aβ₂₉₋₃₃r) were two fold greater than that of the original peptides while the Ki values for the polar forms (Aβ₁₇₋₂₁p and Aβ₂₉₋₃₃p) were between 3-4 fold greater than that of the original peptides. It was also found that Ag nps (Ki = 0.12 μM) inhibited the activity of nNOS the most compared to Au nps; (Ki = 0.15 μM) and curcumin (Ki = 0.25 μM). At 298K, all the ligands bound at a single site on the enzyme (n=1) and a single Trp residue (θ =1), (later identified as Trp678) was made available on the enzyme surface for quenching by the ligands. Increasing the temperature from 298K-313K, increased the value of Ksv and pointed to a dynamic quenching mechanism for Aβ peptides, nps and curcumin interaction with nNOS. The positive signs for entropy and enthalpy for all Aβ peptides nps and curcumin pointed to hydrophobic–hydrophobic interaction with the enzyme. The fact that Kd increased with temperature emphasized the endothermic nature of the binding reaction and the requirement of thermal energy to aid in diffusion of the ligand to the active site. It was concluded that the binding reaction between the ligands and nNOS was non-spontaneous and endothermic at low temperatures (+ΔG) but spontaneous at high temperatures (-ΔG). The two amino acids Tyr706 and Trp678 moved from their original positions, subject to ligand binding. Trp678 moved a minimum distance of 5 Å toward the heme while Tyr706 moved a maximum distance of 14 Å away from the heme. AutoDock 4.2 was a valuable tool in monitoring the distance of Trp678 within the enzyme interior and fluorescence resonance energy transfer (FRET) was efficient in monitoring the distance moved by Trp residues on the enzyme surface.
- Full Text:
- Date Issued: 2014
Biofuel, land-use tradeoffs and livelihoods in Southern Africa
- Authors: Von Maltitz, Graham Paul
- Date: 2014
- Subjects: Biomass energy -- Climatic factors -- Africa, Southern , Land use -- Environmental aspects , Jatropha -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10643 , http://hdl.handle.net/10948/d1021005
- Description: The rapid expansion of biofuel projects in southern Africa creates an opportune issue against which to examine land-use tradeoffs within the areas of customary land tenure. For this an ecosystems services approach is used. Jatropha curcas (L), a perennial oilseed plant which has been the key focus of most of the region’s biofuel expansion to date is used as the focus biofuel crop for which case study data were obtained from Malawi, Mozambique, Zambia and South Africa. Despite the initial enthusiasm for jatropha, most projects have proven less successful than hoped, and many have collapsed. A few are, however, still showing signs of possible success and it is two of these that form the basis of the case studies. Hugely complex tradeoffs are involved when considering biofuel as a land-use option for communal areas. They range from global impacts such as biodiversity and global climate forcing, through national concerns of rural development, national food security and national fuel security, to local household concerns around improving livelihoods. Land that is converted to biofuel needs to be removed from some previous use, and in the southern African case it is typically woodlands and the multitude of services they provide, that suffer. The nature of the tradeoffs and the people affected change over the scale under consideration. For the local farmer it is only the local issues that are of concern, but national and global forces will change the policy environment and lead to new types of development such as biofuels. Change is inevitable, and in all developments there are likely to be both winners and losers. It is clear that the impacts arising from biofuel are situation dependent, and each community and location has unique social and environmental considerations that need to be taken into account. In the case of jatropha the final realised yield and the economic returns that this can generate, will be of critical importance and remain one of the main uncertainties. There are promising signs that under certain circumstances the balance of benefits from jatropha biofuel may be positive, but if implemented incorrectly or in the wrong place, there is extensive evidence of total project failure. It is clear that evidence-based data and assessment tools are needed to assist communities, developers and government departments to make sound decisions around biofuel (or other land-use based) development. A number of such tools are suggested in the thesis. Both the use of large-scale plantations or small-scale farmer centred projects have their advantages and disadvantages. It is probable that in the correct circumstances either can work. However, large-scale plantations can have huge negative social and environmental consequences if poorly implemented. Small-scale projects, though improving livelihoods, are unlikely to take the farmers out of poverty. Tradeoffs from any land-use change are inevitable. Empirical data on biofuel impacts on the environment and society are needed for the development of sound policy. A favourable policy environment can ensure that positive benefits from biofuel are obtained, whilst minimising negative impacts. To develop this policy means that southern African countries will have to clearly understand what they wish to achieve from biofuel, as well as having a clear understanding of impacts from biofuel implementation. Sound scientific knowledge needs to underpin this process. For instance governments may wish to increase the ratio of small-scale to large-scale plantation to increase the developmental benefits, ensure biofuel is used to promote national fuel security rather than being exported, or develop a medium-scale farming sector which can help move farmers out of poverty and assist in developing a market surplus of agricultural commodities. Analysing impacts from biofuel expansion is a complex and multi-dimensional problem and as such will require multi-criteria analysis tools to develop solutions. Global, national and local tradeoffs must all be considered. In addition a wide range of stakeholders are involved and participatory processes may be needed to capture their inputs. Tools to better analyse impacts, specifically at the local level are needed. These local results need to feed into national level economic assessments. The cost of biofuel introduction should be considered against the costs of not implementing biofuel, realising that doing nothing also has a cost and long-term impact. Third-party certification provides a useful tool for shifting costs of ensuring compliance with social and environmental legislation, from the state to biofuel companies. In addition ongoing monitoring and evaluation of existing projects is needed to learn from successes and failures, to identify unintended consequences, and to increase the resilience of projects, community livelihoods and the national economy. This will have to be supplemented with additional focused and ongoing research.
- Full Text:
- Date Issued: 2014
- Authors: Von Maltitz, Graham Paul
- Date: 2014
- Subjects: Biomass energy -- Climatic factors -- Africa, Southern , Land use -- Environmental aspects , Jatropha -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10643 , http://hdl.handle.net/10948/d1021005
- Description: The rapid expansion of biofuel projects in southern Africa creates an opportune issue against which to examine land-use tradeoffs within the areas of customary land tenure. For this an ecosystems services approach is used. Jatropha curcas (L), a perennial oilseed plant which has been the key focus of most of the region’s biofuel expansion to date is used as the focus biofuel crop for which case study data were obtained from Malawi, Mozambique, Zambia and South Africa. Despite the initial enthusiasm for jatropha, most projects have proven less successful than hoped, and many have collapsed. A few are, however, still showing signs of possible success and it is two of these that form the basis of the case studies. Hugely complex tradeoffs are involved when considering biofuel as a land-use option for communal areas. They range from global impacts such as biodiversity and global climate forcing, through national concerns of rural development, national food security and national fuel security, to local household concerns around improving livelihoods. Land that is converted to biofuel needs to be removed from some previous use, and in the southern African case it is typically woodlands and the multitude of services they provide, that suffer. The nature of the tradeoffs and the people affected change over the scale under consideration. For the local farmer it is only the local issues that are of concern, but national and global forces will change the policy environment and lead to new types of development such as biofuels. Change is inevitable, and in all developments there are likely to be both winners and losers. It is clear that the impacts arising from biofuel are situation dependent, and each community and location has unique social and environmental considerations that need to be taken into account. In the case of jatropha the final realised yield and the economic returns that this can generate, will be of critical importance and remain one of the main uncertainties. There are promising signs that under certain circumstances the balance of benefits from jatropha biofuel may be positive, but if implemented incorrectly or in the wrong place, there is extensive evidence of total project failure. It is clear that evidence-based data and assessment tools are needed to assist communities, developers and government departments to make sound decisions around biofuel (or other land-use based) development. A number of such tools are suggested in the thesis. Both the use of large-scale plantations or small-scale farmer centred projects have their advantages and disadvantages. It is probable that in the correct circumstances either can work. However, large-scale plantations can have huge negative social and environmental consequences if poorly implemented. Small-scale projects, though improving livelihoods, are unlikely to take the farmers out of poverty. Tradeoffs from any land-use change are inevitable. Empirical data on biofuel impacts on the environment and society are needed for the development of sound policy. A favourable policy environment can ensure that positive benefits from biofuel are obtained, whilst minimising negative impacts. To develop this policy means that southern African countries will have to clearly understand what they wish to achieve from biofuel, as well as having a clear understanding of impacts from biofuel implementation. Sound scientific knowledge needs to underpin this process. For instance governments may wish to increase the ratio of small-scale to large-scale plantation to increase the developmental benefits, ensure biofuel is used to promote national fuel security rather than being exported, or develop a medium-scale farming sector which can help move farmers out of poverty and assist in developing a market surplus of agricultural commodities. Analysing impacts from biofuel expansion is a complex and multi-dimensional problem and as such will require multi-criteria analysis tools to develop solutions. Global, national and local tradeoffs must all be considered. In addition a wide range of stakeholders are involved and participatory processes may be needed to capture their inputs. Tools to better analyse impacts, specifically at the local level are needed. These local results need to feed into national level economic assessments. The cost of biofuel introduction should be considered against the costs of not implementing biofuel, realising that doing nothing also has a cost and long-term impact. Third-party certification provides a useful tool for shifting costs of ensuring compliance with social and environmental legislation, from the state to biofuel companies. In addition ongoing monitoring and evaluation of existing projects is needed to learn from successes and failures, to identify unintended consequences, and to increase the resilience of projects, community livelihoods and the national economy. This will have to be supplemented with additional focused and ongoing research.
- Full Text:
- Date Issued: 2014
Bioinformatics and data analysis in microbiology:
- Authors: Tastan Bishop, Özlem
- Date: 2014
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/148104 , vital:38710 , ISBN 9781908230737 , https://books.google.co.za/books?id=-G07DwAAQBAJanddq=Bioinformatics+and+data+analysis+in+microbiologyandsource=gbs_navlinks_s
- Description: The rapid advancement of sequencing techniques, coupled with the new methodologies of bioinformatics to handle large-scale data analysis, are providing exciting opportunities for us to understand microbial communities from a variety of environments beyond previous imagination. This book provides invaluable, up-to-date and detailed information on various aspects of bioinformatics data analysis with applications to microbiology. It describes a number of different useful bioinformatics tools, makes links to some wet-lab techniques, explains different approaches to tackle a problem, talks about current challenges and limitations, gives examples of applications of bioinformatics methods to microbiology, and discusses future trends. The chapters include topics such as genome sequencing techniques, assembly, SNP analysis, annotation, comparative genomics, microbial community profiling, metagenomics, phylogenetic microarrays, barcoding and more. Each chapter is written by scientists who are expert in the field, and is peer-reviewed. Bioinformatics and Data Analysis in Microbiology is an essential book for researchers, lecturers and students involved in microbiology, bioinformatics and genome analysis.
- Full Text:
- Date Issued: 2014
- Authors: Tastan Bishop, Özlem
- Date: 2014
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/148104 , vital:38710 , ISBN 9781908230737 , https://books.google.co.za/books?id=-G07DwAAQBAJanddq=Bioinformatics+and+data+analysis+in+microbiologyandsource=gbs_navlinks_s
- Description: The rapid advancement of sequencing techniques, coupled with the new methodologies of bioinformatics to handle large-scale data analysis, are providing exciting opportunities for us to understand microbial communities from a variety of environments beyond previous imagination. This book provides invaluable, up-to-date and detailed information on various aspects of bioinformatics data analysis with applications to microbiology. It describes a number of different useful bioinformatics tools, makes links to some wet-lab techniques, explains different approaches to tackle a problem, talks about current challenges and limitations, gives examples of applications of bioinformatics methods to microbiology, and discusses future trends. The chapters include topics such as genome sequencing techniques, assembly, SNP analysis, annotation, comparative genomics, microbial community profiling, metagenomics, phylogenetic microarrays, barcoding and more. Each chapter is written by scientists who are expert in the field, and is peer-reviewed. Bioinformatics and Data Analysis in Microbiology is an essential book for researchers, lecturers and students involved in microbiology, bioinformatics and genome analysis.
- Full Text:
- Date Issued: 2014
Bioinformatics education—perspectives and challenges out of Africa
- Tastan Bishop, Özlem, Adebiyi, Ezekiel F, Alzohairy, Ahmed M, Everett, Dean B, Ghedira, Kais, Ghouila, Amel, Kumuthini, Judit, Mulder, Nicola J, Panji, Sumir, Patterton, Hugh-G
- Authors: Tastan Bishop, Özlem , Adebiyi, Ezekiel F , Alzohairy, Ahmed M , Everett, Dean B , Ghedira, Kais , Ghouila, Amel , Kumuthini, Judit , Mulder, Nicola J , Panji, Sumir , Patterton, Hugh-G
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123244 , vital:35420 , https://doi.10.1093/bib/bbu022
- Description: The discipline of bioinformatics has developed rapidly since the complete sequencing of the first genomes in the 1990s.The development of many high-throughput techniques during the last decades has ensured that bioinformatics has grown into a discipline that overlaps with, and is required for, the modern practice of virtually every field in the life sciences. This has placed a scientific premium on the availability of skilled bioinformaticians, a qualification that is extremely scarce on the African continent. The reasons for this are numerous, although the absence of a skilled bioinformatician at academic institutions to initiate a training process and build sustained capacity seems to be a common African shortcoming.This dearth of bioinformatics expertise has had a knock-on effect on the establishment of many modern high-throughput projects at African institutes, including the comprehensive and systematic analysis of genomes from African populations, which are among the most genetically diverse anywhere on the planet. Recent funding initiatives from the National Institutes of Health and theWellcomeTrust are aimed at ameliorating this shortcoming. In this paper, we discuss the problems that have limited the establishment of the bioinformatics field in Africa, as well as propose specific actions that will help with the education and training of bioinformaticians on the continent. This is an absolute requirement in anticipation of a boom in high-throughput approaches to human health issues unique to data from African populations.
- Full Text:
- Date Issued: 2014
- Authors: Tastan Bishop, Özlem , Adebiyi, Ezekiel F , Alzohairy, Ahmed M , Everett, Dean B , Ghedira, Kais , Ghouila, Amel , Kumuthini, Judit , Mulder, Nicola J , Panji, Sumir , Patterton, Hugh-G
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123244 , vital:35420 , https://doi.10.1093/bib/bbu022
- Description: The discipline of bioinformatics has developed rapidly since the complete sequencing of the first genomes in the 1990s.The development of many high-throughput techniques during the last decades has ensured that bioinformatics has grown into a discipline that overlaps with, and is required for, the modern practice of virtually every field in the life sciences. This has placed a scientific premium on the availability of skilled bioinformaticians, a qualification that is extremely scarce on the African continent. The reasons for this are numerous, although the absence of a skilled bioinformatician at academic institutions to initiate a training process and build sustained capacity seems to be a common African shortcoming.This dearth of bioinformatics expertise has had a knock-on effect on the establishment of many modern high-throughput projects at African institutes, including the comprehensive and systematic analysis of genomes from African populations, which are among the most genetically diverse anywhere on the planet. Recent funding initiatives from the National Institutes of Health and theWellcomeTrust are aimed at ameliorating this shortcoming. In this paper, we discuss the problems that have limited the establishment of the bioinformatics field in Africa, as well as propose specific actions that will help with the education and training of bioinformaticians on the continent. This is an absolute requirement in anticipation of a boom in high-throughput approaches to human health issues unique to data from African populations.
- Full Text:
- Date Issued: 2014
Biological control as an integrated control method in the management of aquatic weeds in an urban environmental and socio-political landscape : case study : Cape Town Metropolitan Area
- Authors: Stafford, Martha Louise
- Date: 2014
- Subjects: Aquatic weeds -- South Africa -- Cape Town , Aquatic weeds -- Biological control -- South Africa -- Cape Town , Water hyacinth -- Biological control -- South Africa -- Cape Town , Metropolitan areas -- South Africa -- Cape Town
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5874 , http://hdl.handle.net/10962/d1013015
- Description: Aquatic weeds transform and degrade the ecosystems which they invade, impacting various aspects of their surroundings ranging from the community level to disrupting important processes affecting ecosystem services. All of the major aquatic weeds of South Africa are found in the Cape Town Metropolitan Area. Landowners, whether private or public, are legally obliged to manage the listed invasive species through applying environmentally acceptable methodologies. This thesis provides an overview of the strategic management options, prevention, early detection, rapid response and eradication of new invasions, and containment and control species of established species. It discusses the different control methods available for managing aquatic weeds, namely mechanical, manual, chemical and biological, and the integration of different methods to improve their effectiveness. Although various studies have shown that biological control is the most cost–effective, environmentally-friendly and sustainable method, it is not yet fully integrated into weed management programmes in South Africa. In addition, the successes achieved in other parts of the world with the control of water hyacinth through biological control have not been repeated in the urban environment, despite the fact that South Africa has the highest number of biological control agents available for the weed. Urbanisation puts pressure on the natural environment and ecosystem functioning. Nutrient-enriched waters support aquatic weed growth and pose a challenge to the management thereof, in particular with regard to integrating biological control into management programmes. The aims of this study were to determine the reasons for the lack of integration of biological control into weed management programmes in South Africa, to determine the feasibility of integrating biological control in aquatic weed management programmes in a complex urban environmental and socio-political landscape by means of three case studies in the Cape Town Metropolitan Area, which showed that biological control is feasible in urban environments and should be considered. Two surveys were conducted to determine the reasons for the lack of integration of biological control into weed management programmes. The surveys showed that there is a gap between research and implementation as a result of poor communication, non-supporting institutional arrangements and a lack of appropriate capacity and skills at the implementation level. Recommendations were offered to address these issues.
- Full Text:
- Date Issued: 2014
- Authors: Stafford, Martha Louise
- Date: 2014
- Subjects: Aquatic weeds -- South Africa -- Cape Town , Aquatic weeds -- Biological control -- South Africa -- Cape Town , Water hyacinth -- Biological control -- South Africa -- Cape Town , Metropolitan areas -- South Africa -- Cape Town
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5874 , http://hdl.handle.net/10962/d1013015
- Description: Aquatic weeds transform and degrade the ecosystems which they invade, impacting various aspects of their surroundings ranging from the community level to disrupting important processes affecting ecosystem services. All of the major aquatic weeds of South Africa are found in the Cape Town Metropolitan Area. Landowners, whether private or public, are legally obliged to manage the listed invasive species through applying environmentally acceptable methodologies. This thesis provides an overview of the strategic management options, prevention, early detection, rapid response and eradication of new invasions, and containment and control species of established species. It discusses the different control methods available for managing aquatic weeds, namely mechanical, manual, chemical and biological, and the integration of different methods to improve their effectiveness. Although various studies have shown that biological control is the most cost–effective, environmentally-friendly and sustainable method, it is not yet fully integrated into weed management programmes in South Africa. In addition, the successes achieved in other parts of the world with the control of water hyacinth through biological control have not been repeated in the urban environment, despite the fact that South Africa has the highest number of biological control agents available for the weed. Urbanisation puts pressure on the natural environment and ecosystem functioning. Nutrient-enriched waters support aquatic weed growth and pose a challenge to the management thereof, in particular with regard to integrating biological control into management programmes. The aims of this study were to determine the reasons for the lack of integration of biological control into weed management programmes in South Africa, to determine the feasibility of integrating biological control in aquatic weed management programmes in a complex urban environmental and socio-political landscape by means of three case studies in the Cape Town Metropolitan Area, which showed that biological control is feasible in urban environments and should be considered. Two surveys were conducted to determine the reasons for the lack of integration of biological control into weed management programmes. The surveys showed that there is a gap between research and implementation as a result of poor communication, non-supporting institutional arrangements and a lack of appropriate capacity and skills at the implementation level. Recommendations were offered to address these issues.
- Full Text:
- Date Issued: 2014
Biological evaluation and semi-synthesis of Isolated compounds from (Syzygium aromaticum (L.) Merr. & Perry) Buds
- Authors: Sibusiso, Rali
- Date: 2014
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11352 , http://hdl.handle.net/10353/d1021140
- Description: Natural products play a fundamental role in modern drug discovery as they continue providing diverse bioactive lead compounds for new drug formulation. However, isolation of these valued compounds is problematic. On the other hand, the morbidity and mortality rates caused by non-communicable diseases are increasing with improved longevity. Thus, the study herein focused on the isolation of plant-derived compounds from Syzygium aromaticum and evaluated their biological properties. Syzygium aromaticum is a well-known plant which belongs to family Myrtaceae. Dried flower buds of S. aromaticum were subjected to sequential solvent extraction. The ethyl acetate extract (15.535 g) was subjected to column chromatography using a silica gel (0.063-0.200 mm) for isolation. This has led to the isolation of three distinct compounds that were identified as eugenol, maslinic acid (MA) and oleanolic acid (OA). The structural elucidation of these valued compounds was done using NMR, GC-MS, LC-MS, FT-IR and Mp. Further semi-synthesis of the oleanolic acid afforded acetate and ester OA-derived compounds with better solubility properties. All these compounds were evaluated for analgesic and anti-inflammatory properties. All tested compounds were administered at a dose of 40 mg/kg to both Wistar rats and Swiss mice. Significant (p<0.01) analgesic and anti-inflammatory properties are obtained for all compounds. The effects of eugenol in all experiment were better except in the thermal-induced pain or tail flick test. In the case of modified compounds, the formalin induced pain test disclosed that oleanane derived compounds confessed analgesic and anti-inflammatory effects better than OA, whereas, in tale flick test oleanolic acid proved superior analgesic effects compared to all its derivatives with the exception of the acetyl-derivative. Acute anti-inflammatory test showed that acetyl-derivatives were more active than other compounds. In conclusion, chromatographic techniques and semi-synthesise of oleanolic acid have resulted to several plant-derived compounds with analgesic and anti-inflammatory properties. The semi-synthesized compounds may serve as alternative drug candidates for new analgesic and anti-inflammatory drug formulation
- Full Text:
- Date Issued: 2014
- Authors: Sibusiso, Rali
- Date: 2014
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11352 , http://hdl.handle.net/10353/d1021140
- Description: Natural products play a fundamental role in modern drug discovery as they continue providing diverse bioactive lead compounds for new drug formulation. However, isolation of these valued compounds is problematic. On the other hand, the morbidity and mortality rates caused by non-communicable diseases are increasing with improved longevity. Thus, the study herein focused on the isolation of plant-derived compounds from Syzygium aromaticum and evaluated their biological properties. Syzygium aromaticum is a well-known plant which belongs to family Myrtaceae. Dried flower buds of S. aromaticum were subjected to sequential solvent extraction. The ethyl acetate extract (15.535 g) was subjected to column chromatography using a silica gel (0.063-0.200 mm) for isolation. This has led to the isolation of three distinct compounds that were identified as eugenol, maslinic acid (MA) and oleanolic acid (OA). The structural elucidation of these valued compounds was done using NMR, GC-MS, LC-MS, FT-IR and Mp. Further semi-synthesis of the oleanolic acid afforded acetate and ester OA-derived compounds with better solubility properties. All these compounds were evaluated for analgesic and anti-inflammatory properties. All tested compounds were administered at a dose of 40 mg/kg to both Wistar rats and Swiss mice. Significant (p<0.01) analgesic and anti-inflammatory properties are obtained for all compounds. The effects of eugenol in all experiment were better except in the thermal-induced pain or tail flick test. In the case of modified compounds, the formalin induced pain test disclosed that oleanane derived compounds confessed analgesic and anti-inflammatory effects better than OA, whereas, in tale flick test oleanolic acid proved superior analgesic effects compared to all its derivatives with the exception of the acetyl-derivative. Acute anti-inflammatory test showed that acetyl-derivatives were more active than other compounds. In conclusion, chromatographic techniques and semi-synthesise of oleanolic acid have resulted to several plant-derived compounds with analgesic and anti-inflammatory properties. The semi-synthesized compounds may serve as alternative drug candidates for new analgesic and anti-inflammatory drug formulation
- Full Text:
- Date Issued: 2014
Biospeciation and antidiabetic effects of oxidovanadium(IV) complexes
- Authors: Ugirinema, Vital
- Date: 2014
- Subjects: Vanadium compounds , Diabetes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10442 , http://hdl.handle.net/10948/d1020973
- Description: The syntheses of bis(1R-imidazole-2/4-carboxylato)oxidovanadium(IV) complexes was successfully carried out and the complexes were isolated in the solid state. The coordinated water was confirmed by elemental analyses, and single crystal XRD. The complexes were therefore distorted octahedral rather than square planar due to the coordination of water at the sixth position. The reaction of the vanadyl ion (VO2+) with imidazole-4-carboxylic acid (Im4COOH), imidazole-2-carboxylic acid (Im2COOH) and methylimidazole-2-carboxylic acid (MeIm2COOH), respectively, in the presence of small bioligands (bL) [oxalate (Ox), lactate (Lact), and phosphate (Phos)] and high molecular weight (HMM) human serum proteins [albumin (HSA) and transferrin (hTf)] were studied in aqueous solution using potentiometric acid base titrations under oxygen and carbon dioxide–free conditions. The data obtained from these titrations was used to calculate the binary and ternary stability constants using the programme HYPERQUAD. The overall stability constants for VO2+-L-Ox system (log β1111 = 18.9, 18.79 and 19.86), VO2+-L-Lact system (log β1111 = 21.83, 20.98 and 22.86), and VO2+-L-Phos system (log β1111 = 27.35, 24.16 and 27.42) (for L= Im4COOH, Im2COOH and MeIm2COOH, respectively) were obtained. The species distribution diagrams showed that under physiological pH the following ternary and quaternary species; [(VO)L(bL)], and [VO(L)(bL)(OH)], would dominate provided that the competition with serum proteins is not too strong. These species were also confirmed by HPLC, LC-MS and EPR. The overall stability constants for the VO2+-L-HSA system (log β2,1,1,0 = 24.3, 23.7 and 24.7), and for the VO2+-L-hTf system (log β2,2,1,0 = 31.1, 30.8, 36.4 for L = Im4COOH, Im2COOH and MeIm2COOH, respectively), suggesting stronger binding of transferrin. The formation constants for the formation of binary (VO(IV) and the proteins) were 9.1 and 13 for log β11, and 20.9 and 25.2 for β12, for human serum albumin and human serum transferrin respectively. The species distribution diagrams for the proteins (HMM) with oxidovanadium(IV) under physiological pH was dominated by VO(HMM)2, VOL(HMM) for unsubstituted Im4COOH and Im2COOH, however, for the N-substituted MeIm2COOH, the species distribution diagrams under physiological pH, were dominated by VOL2, VO(HMM)2 and VO2L2(HMM). These species were further confirmed by HPLC, MALDI-TOF-MS and EPR. The glucose stimulated insulin secretion (GSIS) action of the complexes was investigated using INS-1E cells at 1μM concentration which was established through cytotoxicity studies via the MTT assay. The vanadium salt (VOSO4), cationic vanadium(IV) complex ([VO(MeImCH2OH)2]2+) were also included in the GSIS study in addition to the three neutral complexes [VO(Im4COO)2, VO(Im2COO)2 and VO(MeIm2COO)2] for comparison. The neutral complexes, especially VO(MeIm2COO)2, showed promising results in the stimulation of insulin secretion than the cationic complex and the vanadium salt.
- Full Text:
- Date Issued: 2014
- Authors: Ugirinema, Vital
- Date: 2014
- Subjects: Vanadium compounds , Diabetes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10442 , http://hdl.handle.net/10948/d1020973
- Description: The syntheses of bis(1R-imidazole-2/4-carboxylato)oxidovanadium(IV) complexes was successfully carried out and the complexes were isolated in the solid state. The coordinated water was confirmed by elemental analyses, and single crystal XRD. The complexes were therefore distorted octahedral rather than square planar due to the coordination of water at the sixth position. The reaction of the vanadyl ion (VO2+) with imidazole-4-carboxylic acid (Im4COOH), imidazole-2-carboxylic acid (Im2COOH) and methylimidazole-2-carboxylic acid (MeIm2COOH), respectively, in the presence of small bioligands (bL) [oxalate (Ox), lactate (Lact), and phosphate (Phos)] and high molecular weight (HMM) human serum proteins [albumin (HSA) and transferrin (hTf)] were studied in aqueous solution using potentiometric acid base titrations under oxygen and carbon dioxide–free conditions. The data obtained from these titrations was used to calculate the binary and ternary stability constants using the programme HYPERQUAD. The overall stability constants for VO2+-L-Ox system (log β1111 = 18.9, 18.79 and 19.86), VO2+-L-Lact system (log β1111 = 21.83, 20.98 and 22.86), and VO2+-L-Phos system (log β1111 = 27.35, 24.16 and 27.42) (for L= Im4COOH, Im2COOH and MeIm2COOH, respectively) were obtained. The species distribution diagrams showed that under physiological pH the following ternary and quaternary species; [(VO)L(bL)], and [VO(L)(bL)(OH)], would dominate provided that the competition with serum proteins is not too strong. These species were also confirmed by HPLC, LC-MS and EPR. The overall stability constants for the VO2+-L-HSA system (log β2,1,1,0 = 24.3, 23.7 and 24.7), and for the VO2+-L-hTf system (log β2,2,1,0 = 31.1, 30.8, 36.4 for L = Im4COOH, Im2COOH and MeIm2COOH, respectively), suggesting stronger binding of transferrin. The formation constants for the formation of binary (VO(IV) and the proteins) were 9.1 and 13 for log β11, and 20.9 and 25.2 for β12, for human serum albumin and human serum transferrin respectively. The species distribution diagrams for the proteins (HMM) with oxidovanadium(IV) under physiological pH was dominated by VO(HMM)2, VOL(HMM) for unsubstituted Im4COOH and Im2COOH, however, for the N-substituted MeIm2COOH, the species distribution diagrams under physiological pH, were dominated by VOL2, VO(HMM)2 and VO2L2(HMM). These species were further confirmed by HPLC, MALDI-TOF-MS and EPR. The glucose stimulated insulin secretion (GSIS) action of the complexes was investigated using INS-1E cells at 1μM concentration which was established through cytotoxicity studies via the MTT assay. The vanadium salt (VOSO4), cationic vanadium(IV) complex ([VO(MeImCH2OH)2]2+) were also included in the GSIS study in addition to the three neutral complexes [VO(Im4COO)2, VO(Im2COO)2 and VO(MeIm2COO)2] for comparison. The neutral complexes, especially VO(MeIm2COO)2, showed promising results in the stimulation of insulin secretion than the cationic complex and the vanadium salt.
- Full Text:
- Date Issued: 2014
Blind ear
- Authors: Berold, Robert
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , poem
- Identifier: http://hdl.handle.net/10962/461973 , vital:76256 , ISBN 0028-4459 , https://journals.co.za/doi/epdf/10.10520/EJC171519
- Description: New Coin is one of South Africa's most established and influential poetry journals. It publishes poetry, and poetry-related reviews, commentary and interviews. New Coin places a particular emphasis on evolving forms and experimental use of the English language in poetry in the South African context. In this sense it has traced the most exciting trends and currents in contemporary poetry in South Africa for a decade of more. The journal is published twice a year in June and December by the Institute for the Study of English in Africa (ISEA), Rhodes University.
- Full Text:
- Date Issued: 2014
- Authors: Berold, Robert
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , poem
- Identifier: http://hdl.handle.net/10962/461973 , vital:76256 , ISBN 0028-4459 , https://journals.co.za/doi/epdf/10.10520/EJC171519
- Description: New Coin is one of South Africa's most established and influential poetry journals. It publishes poetry, and poetry-related reviews, commentary and interviews. New Coin places a particular emphasis on evolving forms and experimental use of the English language in poetry in the South African context. In this sense it has traced the most exciting trends and currents in contemporary poetry in South Africa for a decade of more. The journal is published twice a year in June and December by the Institute for the Study of English in Africa (ISEA), Rhodes University.
- Full Text:
- Date Issued: 2014
Book Review: Community of insecurity
- Authors: Magadla, Siphokazi
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298606 , vital:57720 , xlink:href="https://doi.org/10.1080/02589001.2014.936174"
- Description: Laurie Nathan's Community of Insecurity is a thoughtful engagement with the ‘establishment, evolution and effectiveness of the regional security arrangements of the Southern African Development Community (SADC)’ (1). He argues that it is of ‘great significance and irony that many of the major disputes among member states have been around the orientation and strategies of peacemaking and regional security’. Nathan's empirical claims about the SADC security architecture are drawn from his ‘insider perspective’ due to his involvement in official efforts to design a common security regime in Southern Africa, which include his service as regional security advisor to the SADC Secretariat from 1992 to 1996, to the Foreign Minister of Mozambique Pascoal Mocumbi from 1994 to 1995, to the South African Minister of Defence Joe Modise and the Deputy Minister of Defence Ronnie Kasrils between 1994 and 1999 and to the Foreign Minister of Swaziland Albert Shabangu when he oversaw the SADC Organ on Politics, Defence and Security Cooperation in 1999–2000 (12–13). This ‘insider’ view has allowed him to make a compelling challenge to current theoretical debates about SADC's status as a security community, that is, a community with an affinity amongst member states that is ‘so strong that they enjoy dependable expectations of peaceful change and thus regard the prospect of war among them as inconceivable’(129). While other commentators see SADC as either a ‘nascent’, ‘embryonic’ or ‘emerging’ security community, Nathan argues forcefully that ‘this perspective is mistaken’ as SADC continues to be demonstrative of a what he describes as a ‘community of insecurity’.
- Full Text:
- Date Issued: 2014
- Authors: Magadla, Siphokazi
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298606 , vital:57720 , xlink:href="https://doi.org/10.1080/02589001.2014.936174"
- Description: Laurie Nathan's Community of Insecurity is a thoughtful engagement with the ‘establishment, evolution and effectiveness of the regional security arrangements of the Southern African Development Community (SADC)’ (1). He argues that it is of ‘great significance and irony that many of the major disputes among member states have been around the orientation and strategies of peacemaking and regional security’. Nathan's empirical claims about the SADC security architecture are drawn from his ‘insider perspective’ due to his involvement in official efforts to design a common security regime in Southern Africa, which include his service as regional security advisor to the SADC Secretariat from 1992 to 1996, to the Foreign Minister of Mozambique Pascoal Mocumbi from 1994 to 1995, to the South African Minister of Defence Joe Modise and the Deputy Minister of Defence Ronnie Kasrils between 1994 and 1999 and to the Foreign Minister of Swaziland Albert Shabangu when he oversaw the SADC Organ on Politics, Defence and Security Cooperation in 1999–2000 (12–13). This ‘insider’ view has allowed him to make a compelling challenge to current theoretical debates about SADC's status as a security community, that is, a community with an affinity amongst member states that is ‘so strong that they enjoy dependable expectations of peaceful change and thus regard the prospect of war among them as inconceivable’(129). While other commentators see SADC as either a ‘nascent’, ‘embryonic’ or ‘emerging’ security community, Nathan argues forcefully that ‘this perspective is mistaken’ as SADC continues to be demonstrative of a what he describes as a ‘community of insecurity’.
- Full Text:
- Date Issued: 2014
Bosman as Verbindingsteken: Hybridities in the Writing of Herman Charles Bosman.
- Authors: Leff, Carol Willa
- Date: 2014
- Subjects: Bosman, Herman Charles, 1905-1951 -- Criticism and interpretation , South African fiction (English) -- 20th century -- History and criticism , Authors, South African -- 20th century , South Africa -- Social life and customs -- 20th century , National characteristics, South African
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2314 , http://hdl.handle.net/10962/d1013163
- Description: This thesis is concerned with how hybridity is created and interpreted by Herman Charles Bosman in his fiction and non-fiction. Bosman was a gifted writer and raconteur who captured the historical, socio-political context of his time by translating Afrikaans culture for the edification and pleasure of an English readership. Hennie Aucamp summed up this linguistic and cultural translation by pointing out that Bosman was a writer who acted as a “verbindingsteken” or hyphen (65) between Afrikaans and English. His texts contain many voices, and are therefore essentially hybrid. Firstly, by drawing on aspects of postcolonial theory, the terms ‘hybridity’, ‘culture’ and ‘identity’, are discussed. Homi Bhabha’s notion of ‘hybridity’ is the conceptual lens through which Bosman’s texts are viewed, and aspects of Mikhail Bakhtin’s cultural theory also serve the same function. Thereafter, biographies of Bosman are discussed in an effort to understand his hyphenated identity. Following this, specific attention is paid to a selection of Bosman’s essays, short stories, and a novel. Scholarly opinions aid interpretation of levels of hybridity in Bosman’s work. In analysing Bosman’s texts critically, it becomes clear that he believed in a united South Africa that acknowledged and accepted all races. However, analysis also reveals that there are some inconsistencies in Bosman’s personal views, as expressed particularly in his essays. His short stories do not contain the same contradictions. Critical analysis of the novel Willemsdorp attests that cultural hybridity is not always viewed as celebratory. It can also be a painful space where identities are split, living both inside and outside their environment, and subsequently marginalized. Bosman’s texts, although published decades ago, remain relevant today in post-apartheid South Africa as much of his writing can be seen as a record of historical events. His short stories and novels capture a confluence of languages, people and cultures. His essays illustrate a deep commitment to promoting South African culture and literature. When reading Bosman one is constantly reminded that differences are not only to be acknowledged, but embraced, in what he prophetically imagined as a hybrid, post-apartheid South African society.
- Full Text:
- Date Issued: 2014
- Authors: Leff, Carol Willa
- Date: 2014
- Subjects: Bosman, Herman Charles, 1905-1951 -- Criticism and interpretation , South African fiction (English) -- 20th century -- History and criticism , Authors, South African -- 20th century , South Africa -- Social life and customs -- 20th century , National characteristics, South African
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2314 , http://hdl.handle.net/10962/d1013163
- Description: This thesis is concerned with how hybridity is created and interpreted by Herman Charles Bosman in his fiction and non-fiction. Bosman was a gifted writer and raconteur who captured the historical, socio-political context of his time by translating Afrikaans culture for the edification and pleasure of an English readership. Hennie Aucamp summed up this linguistic and cultural translation by pointing out that Bosman was a writer who acted as a “verbindingsteken” or hyphen (65) between Afrikaans and English. His texts contain many voices, and are therefore essentially hybrid. Firstly, by drawing on aspects of postcolonial theory, the terms ‘hybridity’, ‘culture’ and ‘identity’, are discussed. Homi Bhabha’s notion of ‘hybridity’ is the conceptual lens through which Bosman’s texts are viewed, and aspects of Mikhail Bakhtin’s cultural theory also serve the same function. Thereafter, biographies of Bosman are discussed in an effort to understand his hyphenated identity. Following this, specific attention is paid to a selection of Bosman’s essays, short stories, and a novel. Scholarly opinions aid interpretation of levels of hybridity in Bosman’s work. In analysing Bosman’s texts critically, it becomes clear that he believed in a united South Africa that acknowledged and accepted all races. However, analysis also reveals that there are some inconsistencies in Bosman’s personal views, as expressed particularly in his essays. His short stories do not contain the same contradictions. Critical analysis of the novel Willemsdorp attests that cultural hybridity is not always viewed as celebratory. It can also be a painful space where identities are split, living both inside and outside their environment, and subsequently marginalized. Bosman’s texts, although published decades ago, remain relevant today in post-apartheid South Africa as much of his writing can be seen as a record of historical events. His short stories and novels capture a confluence of languages, people and cultures. His essays illustrate a deep commitment to promoting South African culture and literature. When reading Bosman one is constantly reminded that differences are not only to be acknowledged, but embraced, in what he prophetically imagined as a hybrid, post-apartheid South African society.
- Full Text:
- Date Issued: 2014
Breast cancer: current developments in molecular approaches to diagnosis and treatment
- de la Mare, Jo-Anne, Contu, Lara, Hunter, Morgan C, Moyo, Buhle, Sterrenberg, Jason N, Dhanani, Karim C H, Mutsvunguma, Lorraine Z, Edkins, Adrienne L
- Authors: de la Mare, Jo-Anne , Contu, Lara , Hunter, Morgan C , Moyo, Buhle , Sterrenberg, Jason N , Dhanani, Karim C H , Mutsvunguma, Lorraine Z , Edkins, Adrienne L
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164819 , vital:41175 , DOI: 10.2174/15748928113086660046
- Description: Due to the high heterogeneity of breast cancers, numerous recent patents describe improved methods of detection and classification which promise better patient prognosis and treatment. In particular, there has been a shift towards more effective genetic screening to identify specific mutations associated with breast tumours, which may lead to “personalised medicine” with improved outcomes. Two challenging areas of breast cancer research involve the development of treatments for the highly aggressive triple negative breast cancer subtype as well as the chemotherapy-resistant cancer stem cell subpopulation. In addition, despite numerous recent advances in breast cancer treatment in woman, male breast cancer remains poorly understood and there are limited therapies available which are developed specifically for men. This review serves to report on important developments in the treatment of breast malignancies patented in the past two years as well as to highlight the current gaps in the field of breast cancer therapeutics and areas which require further study.
- Full Text:
- Date Issued: 2014
- Authors: de la Mare, Jo-Anne , Contu, Lara , Hunter, Morgan C , Moyo, Buhle , Sterrenberg, Jason N , Dhanani, Karim C H , Mutsvunguma, Lorraine Z , Edkins, Adrienne L
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164819 , vital:41175 , DOI: 10.2174/15748928113086660046
- Description: Due to the high heterogeneity of breast cancers, numerous recent patents describe improved methods of detection and classification which promise better patient prognosis and treatment. In particular, there has been a shift towards more effective genetic screening to identify specific mutations associated with breast tumours, which may lead to “personalised medicine” with improved outcomes. Two challenging areas of breast cancer research involve the development of treatments for the highly aggressive triple negative breast cancer subtype as well as the chemotherapy-resistant cancer stem cell subpopulation. In addition, despite numerous recent advances in breast cancer treatment in woman, male breast cancer remains poorly understood and there are limited therapies available which are developed specifically for men. This review serves to report on important developments in the treatment of breast malignancies patented in the past two years as well as to highlight the current gaps in the field of breast cancer therapeutics and areas which require further study.
- Full Text:
- Date Issued: 2014
Building Information Modelling as a catalyst for an Integrated Construction Project Delivery culture in South Africa
- Authors: Froise, Timothy
- Date: 2014
- Subjects: Construction industry -- South Africa , Construction projects -- South Africa , Acculturation -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9716 , http://hdl.handle.net/10948/d1020872
- Description: The importance of technology in construction partnering agreements has been recognised as a vital part of integrated project delivery (IPD) philosophy. Building information modelling (BIM) is increasingly being used by consultants in South Africa during the design phase of construction projects. However, its use is generally not aimed at an integrated project delivery approach, but rather as a tool to generate documentation, as BIM is generally more efficient than traditional computer aided design (CAD) software. For the full benefits of BIM to be realised, a greater degree of acculturation is required between construction project organisations (CPOs). The current cultures of CPOs are separationist in nature and tend to inhibit acculturation within the industry. BIM provides a central source of information that can improve communications between CPOs and foster a collaborative culture. The research examines IPD and BIM in the South African context and investigates how BIM can contribute to IPD. A survey was conducted among registered contractors from the three top grades and architecture practices from two regions in South Africa. The survey was placed in context by a case study that analysed the use of BIM and the resulting communication network seen in a public works project using typical procurement methods. The associated problems with the current accepted paradigm are illuminated by the research.
- Full Text:
- Date Issued: 2014
- Authors: Froise, Timothy
- Date: 2014
- Subjects: Construction industry -- South Africa , Construction projects -- South Africa , Acculturation -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9716 , http://hdl.handle.net/10948/d1020872
- Description: The importance of technology in construction partnering agreements has been recognised as a vital part of integrated project delivery (IPD) philosophy. Building information modelling (BIM) is increasingly being used by consultants in South Africa during the design phase of construction projects. However, its use is generally not aimed at an integrated project delivery approach, but rather as a tool to generate documentation, as BIM is generally more efficient than traditional computer aided design (CAD) software. For the full benefits of BIM to be realised, a greater degree of acculturation is required between construction project organisations (CPOs). The current cultures of CPOs are separationist in nature and tend to inhibit acculturation within the industry. BIM provides a central source of information that can improve communications between CPOs and foster a collaborative culture. The research examines IPD and BIM in the South African context and investigates how BIM can contribute to IPD. A survey was conducted among registered contractors from the three top grades and architecture practices from two regions in South Africa. The survey was placed in context by a case study that analysed the use of BIM and the resulting communication network seen in a public works project using typical procurement methods. The associated problems with the current accepted paradigm are illuminated by the research.
- Full Text:
- Date Issued: 2014
Business school creativity amongst MBA students at Nelson Mandela Metropolitan University
- Authors: Von Solms, Woudi
- Date: 2014
- Subjects: Creative ability in business , Creative thinking , Educational innovations
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/4255 , vital:20574
- Description: Innovation allows for competitive advantage. Competitive advantage and innovation lead to economic growth. For innovation to occur, creativity is necessary. All individuals are creative, but continuous practice is necessary to be creative. The process from creativity to innovation and competitive advantage and economic growth involves three stages. The first stage involves educating primary and secondary school children to be creative across different domains. Current school systems focus on mathematical and linguistic skills. Examples of domains are linguistic, performance, mechanicalscientific and artistic. These creative domains can be taught through encouraging children to use their imagination and different methods. In primary and secondary education little-c creativity and mini-c creativity are developed. Little-c creativity involves developing problem solving skills. Mini-c creativity involves the ability to recognise personal creative events, not necessarily recognised by others. Tertiary education involves students gaining knowledge in a specific domain. While studying to gain knowledge and conduct research on a specific domain, practicing creativity is still important. Upon completion of tertiary education the second stage in creating economic growth through creativity and innovation commences. This second stage involves organisations hiring creative employees that have knowledge within a domain similar to the organisation‟s industry. Creative employees develop creative ideas. The creative ideas allow for innovative products to be developed. Innovative products satisfy customer needs and lead to competitive advantage. Managers should encourage employees to be innovative. Employees that are motivated and encouraged to take risks develop a talent within the domain that they are employed in. The ability to be creative and innovative leads to pro-creativity and big-c creativity. Pro-c creativity refers to a talent being developed over approximately ten years. Big-c creativity involves creating products that benefits society positively. Upon creating products that benefit society, competitive advantage is created that allows for the third stage of creating economic growth to commence. This third stage involves economic growth that stems from innovation and creative individuals. Countries currently focus on stage two where they aim to encourage innovation amongst organisations. There is a realisation that to be innovative,creativity is necessarily and that creativity must be taught by means of education. This research study will use a questionnaire by Kaufman (2009, 2012, 2013) to determine how creative students perceive they are and whether they are capable of recognising different levels of creativity. A similar study was conducted in Germany and Mexico. Professor Alexander Brem was asked for the questionnaire. The questionnaire was translated from German to English. The objective is to determine whether students perceive themselves to be creative, thus being innovative within their organisations and indirectly allowing for competitive advantage and economic growth. Kaufmans‟ questionnaire was distributed to Nelson Mandela Metropolitan University Masters in Business Administration students. Ethics clearance was granted and answering the questionnaire was optional.To draw conclusions an Exploratory Factor Analysis was done on creative domains and the levels of creativity students are able to recognise. The first Exploratory Factor Analysis revealed performance creativity as the first factor, mechanicalscientific creativity as the second factor, scholarly creativity as the third factor and artistic creativity as the fourth factor. The second Exploratory Factor Analysis grouped pro-c creativity, big-c creativity and little-c creativity as the first factor, not being able to recognise creativity as the second factor and mini-c creativity as the third factor. The individual results from each factor were discussed. Each factor was further analysed by comparing gender, age, year‟s work experience and type of students to the type and level of creativity. The results show three trends. Firstly, results indicated that respondents perceived themselves to be predominantly scholarly and mathematical-scientific creative. This result is synonymous with secondary research that states that educational institutions focus on enhancing linguistic and mathematical skills amongst students and that developing skills in other domains are seen as less important. Secondly, the ability of students to recognise different levels of creativity decreases from big-creativity to pro-c creativity; pro-c creativity to little-c creativity and little-c creativity to mini-c creativity. Students are therefore better able to recognise large inventions than smaller inventions. Thirdly, results indicated that creativity levels do not differ when gender, age, year‟s work experience and type of students are compared. In this research study the above statements will be discussed in detail.
- Full Text:
- Date Issued: 2014
- Authors: Von Solms, Woudi
- Date: 2014
- Subjects: Creative ability in business , Creative thinking , Educational innovations
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/4255 , vital:20574
- Description: Innovation allows for competitive advantage. Competitive advantage and innovation lead to economic growth. For innovation to occur, creativity is necessary. All individuals are creative, but continuous practice is necessary to be creative. The process from creativity to innovation and competitive advantage and economic growth involves three stages. The first stage involves educating primary and secondary school children to be creative across different domains. Current school systems focus on mathematical and linguistic skills. Examples of domains are linguistic, performance, mechanicalscientific and artistic. These creative domains can be taught through encouraging children to use their imagination and different methods. In primary and secondary education little-c creativity and mini-c creativity are developed. Little-c creativity involves developing problem solving skills. Mini-c creativity involves the ability to recognise personal creative events, not necessarily recognised by others. Tertiary education involves students gaining knowledge in a specific domain. While studying to gain knowledge and conduct research on a specific domain, practicing creativity is still important. Upon completion of tertiary education the second stage in creating economic growth through creativity and innovation commences. This second stage involves organisations hiring creative employees that have knowledge within a domain similar to the organisation‟s industry. Creative employees develop creative ideas. The creative ideas allow for innovative products to be developed. Innovative products satisfy customer needs and lead to competitive advantage. Managers should encourage employees to be innovative. Employees that are motivated and encouraged to take risks develop a talent within the domain that they are employed in. The ability to be creative and innovative leads to pro-creativity and big-c creativity. Pro-c creativity refers to a talent being developed over approximately ten years. Big-c creativity involves creating products that benefits society positively. Upon creating products that benefit society, competitive advantage is created that allows for the third stage of creating economic growth to commence. This third stage involves economic growth that stems from innovation and creative individuals. Countries currently focus on stage two where they aim to encourage innovation amongst organisations. There is a realisation that to be innovative,creativity is necessarily and that creativity must be taught by means of education. This research study will use a questionnaire by Kaufman (2009, 2012, 2013) to determine how creative students perceive they are and whether they are capable of recognising different levels of creativity. A similar study was conducted in Germany and Mexico. Professor Alexander Brem was asked for the questionnaire. The questionnaire was translated from German to English. The objective is to determine whether students perceive themselves to be creative, thus being innovative within their organisations and indirectly allowing for competitive advantage and economic growth. Kaufmans‟ questionnaire was distributed to Nelson Mandela Metropolitan University Masters in Business Administration students. Ethics clearance was granted and answering the questionnaire was optional.To draw conclusions an Exploratory Factor Analysis was done on creative domains and the levels of creativity students are able to recognise. The first Exploratory Factor Analysis revealed performance creativity as the first factor, mechanicalscientific creativity as the second factor, scholarly creativity as the third factor and artistic creativity as the fourth factor. The second Exploratory Factor Analysis grouped pro-c creativity, big-c creativity and little-c creativity as the first factor, not being able to recognise creativity as the second factor and mini-c creativity as the third factor. The individual results from each factor were discussed. Each factor was further analysed by comparing gender, age, year‟s work experience and type of students to the type and level of creativity. The results show three trends. Firstly, results indicated that respondents perceived themselves to be predominantly scholarly and mathematical-scientific creative. This result is synonymous with secondary research that states that educational institutions focus on enhancing linguistic and mathematical skills amongst students and that developing skills in other domains are seen as less important. Secondly, the ability of students to recognise different levels of creativity decreases from big-creativity to pro-c creativity; pro-c creativity to little-c creativity and little-c creativity to mini-c creativity. Students are therefore better able to recognise large inventions than smaller inventions. Thirdly, results indicated that creativity levels do not differ when gender, age, year‟s work experience and type of students are compared. In this research study the above statements will be discussed in detail.
- Full Text:
- Date Issued: 2014